| 环介导等温扩增技术检测沙门氏菌反应体系的优化 |
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| 引用本文： | 张胜凯,张先舟,王羽,马晓燕,张会彦,张伟.环介导等温扩增技术检测沙门氏菌反应体系的优化[J].安徽农业科学,2010,38(22):11727-11729. |
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| 作者姓名： | 张胜凯 张先舟 王羽 马晓燕 张会彦 张伟 |
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| 作者单位： | 河北省石家庄市质量技术监督局新华区分局,河北石家庄,050060;河北农业大学食品科技学院,河北保定,071001 |
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| 基金项目： | 河北省自然科学基金项目 |
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| 摘 要： | 目的]建立快速检测沙门氏菌的环介导等温扩增（LAMP）技术。方法]以沙门氏菌的特异性基因（invA）序列为靶序列设计引物,并对该序列进行等温扩增;对Mg2＋浓度、反应温度和反应时间扩增条件进行优化,并对沙门氏菌进行检测和鉴定。结果]在Mg2＋浓度为2.0mmol/L时,62℃反应40min,扩增可达最佳效果,LAMP法检测的灵敏度为7.8cfu/ml。结论]建立了快速检测沙门氏菌的LAMP技术,该方法灵敏度高、耗时短、方法简便,便于在基层推广,为检测食源性致病菌构建了一个技术平台。
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| 关 键 词： | 环介导等温扩增 沙门氏菌 检测 |
Reaction System Optimization for the Detection of Salmonella by Loop-mediated Isothermal Amplification Technology |
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| ZHANG Sheng-kai et al.Reaction System Optimization for the Detection of Salmonella by Loop-mediated Isothermal Amplification Technology[J].Journal of Anhui Agricultural Sciences,2010,38(22):11727-11729. |
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| Authors: | ZHANG Sheng-kai |
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| Institution: | ZHANG Sheng-kai et al (Xinhua Station of Shijiazhuang Quality Technology Surpervision Bureau in Hebei Province,Shijiazhuang,Hebei 050060) |
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| Abstract: | Objective] The research aimed to establish loop-mediated isothermal amplification (LAMP) technology for rapid detection of Salmonella.Method] The primers were designed according to the Salmonella-specific gene (invA) sequence as the target sequence.And the sequence was amplified under the isothermal conditions.The reaction conditions were optimized including Mg2+ concentration,reaction temperature and time etc.And Salmonella stains were detected and identified.Result] The results indicated that the best ... |
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| Keywords: | Loop-mediated isothermal amplification Salmonella Detection |
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