玉米抗丝黑穗病基因SSR分析

目的]筛选出能较好标记玉米抗丝黑穗病基因的SSR多态性引物。方法]以5个玉米品种为试材,从玉米黄化苗提取基因组DNA后,对提取DNA浓度和质量进行检测后,选用85对引物进行抗丝黑穗病基因的SSR扩增。结果]16对引物具有较好的多态性,共扩增出45条多态性谱带,其片段大小为50～1300bp。其中,引物Bnlg1755扩增出多态性谱带最多。引物Bnlg1246、Bnlg1194和Bnlg125在抗病材料和感病材料中表现出明显差异。结论]引物Bnlg1246、Bnlg1194和Bnlg125可作为分析玉米抗丝黑穗病基因SSR标记的引物。

SSR Analysis of Resistant Genes to Head Smut in Maize

Objective] The research aimed to screen out SSR polymorphic primers that could better mark the resistance gene to head smut in maize. Method] With 5 maize varieties as tested materials, genomic DNA was extracted from the etiolated corn seedlings of maize. After the concn. and quality of the extracted DNA was detected, 85 pairs of primers were selected to make SSR amplification on the resistance gene to head smut. Result] Sixteen pairs of primers had better polymorphism and 45 polymorphic bands were amplified, with the fragment size of 50～1300 bp. Among them, the polymorphic bands amplified by primer Bnlg1755 was most. Primers Bnlg1246, Bnlg1194 and Bnlg125 showed an obvious difference between resistant materials and susceptible materials. Conclusion] Bnlg1246、Bnlg1194 Bnlg125 could be taken as the primers of analyzing the SSR markers for the resistance gene to head smut in maize.