UEZC1AS抗真菌作用机理研究

目的]为筛选安全、有效的抗真菌抗生素奠定基础。方法]以黑曲霉和新型隐球菌为供试菌株，以酮康唑、5一Fc和灰黄霉素为对照试剂，用萨氏琼脂培养基培养新型隐球菌，用马铃薯琼脂培养基培养黑曲霉，研究UEZCl代谢合成的抗真菌活性物质UEZClAS的抑茵活性。结果]被UEZClAS抑制的黑曲霉菌丝略膨大变粗，直径4．50～6．45μm，茵丝分支增多；被酮康唑抑制的黑曲霉菌丝膨大变粗，直径3．90～10．40μm，菌丝顶端及分隔处的分支增多，分支多为指形或香蕉形，3～4个聚生于菌丝尖端；被5-FC抑制的黑曲霉菌丝膨大变粗，直径3．90～7．80μm，茵丝的顶端分支增多，分支轮生、极短，呈树枝状。琼脂扩散法抑菌试验发现，新型隐球菌形状不规则，呈非常小的颗粒体。结论]初步推断出UEZClAS的抗真菌作用部位在细胞壁。

Study on the Antifungal Mechanism of UEZC1AS

Objective] The purpose of the study was to lay a foundation for screening safe and effective antifungal antibiotics.Method] With Aspergillus niger and Cryptococcus neoformans as tested strains,ketoconazole,5-FC and grifulvin as control agents,C.neoformans was cultured with Sacharov agar medium and A.niger was cultured with potato agar medium so as to study the antibacterial activity of antifungal active matter UEZC1AS synthesized through the metabolism of UEZC1.Result] The mycelial of A.niger inhibited b...