| 不同培养条件对驱蚊香草愈伤组织诱导的研究 |
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| 引用本文： | 庞发虎,张良,刘飞.不同培养条件对驱蚊香草愈伤组织诱导的研究[J].安徽农业科学,2007,35(3):682-683. |
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| 作者姓名： | 庞发虎 张良 刘飞 |
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| 作者单位： | 南阳师范学院生物系,河南南阳,473061;南阳理工学院,河南南阳,473061 |
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| 基金项目： | 南阳师范学院科研资助项目（nyte2004k10）;河南省南阳市科技局项目（2005PT064）. |
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| 摘 要： | 以驱蚊香草的幼嫩叶片为外植体,应用正交设计法对愈伤组织诱导条件进行研究.结果表明:诱导愈伤组织的最佳培养基配方为:MS+2,4-D2 mg/L+6-BA0.5 mg/L+IBA 0.2 mg/L;外植体用10%的84消毒液消毒10 min效果最好;最适培养温度为20 ℃,最佳光照时间为10 h/d.
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| 关 键 词： | 驱蚊香草 正交设计 愈伤组织 方差分析 |
| 文章编号： | 0517-6611（2007）03-0082-02 |
| 修稿时间： | 2006-08-22 |
Study on the Induction of Callus of Cirosa in Different Culture Conditions |
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| PANG Fa-hu et al.Study on the Induction of Callus of Cirosa in Different Culture Conditions[J].Journal of Anhui Agricultural Sciences,2007,35(3):682-683. |
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| Authors: | PANG Fa-hu |
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| Institution: | Biology Department, Nanyang Normal University, Nanyang, Henan 473061 |
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| Abstract: | With the orthogonalty design,the young leaf of citrosa was used for tissue culture and the induction condition of callus was studied.The result showed that the optimum medium for callus was MS + 2,4-D 2 mg/l + 6-BA 0.5 mg/l + IBA 0.2 mg/l and when the explant was disinfected with 10% of 84 Disinfectant Liquid,it had a better result.The optimum temperature was 20 ℃ and the best light hour was 10 h/d. |
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| Keywords: | Citrosa Orthogonalty design Callus Analysis of variance |
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