| 雷蘑液体发酵工艺优化研究 |
| |
| 引用本文： | 王永斌,王允祥.雷蘑液体发酵工艺优化研究[J].安徽农业科学,2006,34(7):1289-1291. |
| |
| 作者姓名： | 王永斌 王允祥 |
| |
| 作者单位： | 1. 蚌埠学院食品科学与工程系,安徽蚌埠,233000;南京农业大学食品科技学院,江苏南京,210095
2. 蚌埠学院食品科学与工程系,安徽蚌埠,233000;浙江林学院食品与药学学院,浙江临安,311300 |
| |
| 摘 要： | 在雷蘑液体发酵培养基优化的基础上,研究了适于雷蘑深层发酵的初始pH值、接种量、装液量、发酵温度和时间及摇床转速对菌丝生长和胞外多糖产量的影响。并通过L16(45)正交试验对发酵工艺进行了优化,结果表明:在初始pH值6.5,接种量10%,装液量100ml/500 ml,培养温度28℃,培养时间9 d,转速140 r/min的发酵条件下,所得胞外多糖量为93.61 mg/100 ml。
|
| 关 键 词： | 深层培养 雷蘑多糖 正交试验 |
| 文章编号： | 0517-6611（2006）07-1289-03 |
| 收稿时间： | 2006-01-03 |
| 修稿时间： | 2006-01-03 |
Study on the Optimum Process of Clitocybe gigantean AS 5.105 by Submerged Culture |
| |
| WANG Yong-bin et al.Study on the Optimum Process of Clitocybe gigantean AS 5.105 by Submerged Culture[J].Journal of Anhui Agricultural Sciences,2006,34(7):1289-1291. |
| |
| Authors: | WANG Yong-bin |
| |
| Institution: | Department of Food Science, Bengbu College, Bengbu, Anhui 233000 |
| |
| Abstract: | The effect of initial pH,volume of medium,amount of inoculation,temperature,time and on the growth of mycelium and production of exopolysaccharide was studied based on the optimal fermentation medium in submerged cultivation with Clitocybe gigantean AS 5.105.The orthogonal test of L_(16)(4~5) showed the maximum yield of EPC was obtained under the circumstance of volume of medium: 100 ml/500 ml;the amount of inoculation: 10%;pH value: 6.5;rotate speed: 140 r/min;temperature: 28 ℃ and time: 9 days.The maximum yield of EPC was 93.61 mg/100 ml. |
| |
| Keywords: | Submerged culture Clitocybe gigantean polysaccharide Orthogonal test |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
