| 黔产金樱子变型的ISSR分析 |
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| 引用本文： | 王晓多,王晓玲,王晓丽.黔产金樱子变型的ISSR分析[J].安徽农业科学,2014(17):5425-5426. |
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| 作者姓名： | 王晓多 王晓玲 王晓丽 |
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| 作者单位： | 贵阳市花溪区农业局,贵州贵阳,550025;贵州大学,贵州贵阳,550025;贵阳医学院药用植物与生药学教研室,贵州贵阳,550004 |
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| 基金项目： | 黔产大宗药材金樱子、苦参质量评价研究。黔科合ZY字[2012]3004号 |
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| 摘 要： | 目的]揭示贵州境内不同变型金樱子10个居群间的遗传变异情况。方法]采用ISSR分子标记技术，从ISSR随机引物中，筛选有效引物，进行扩增，用NTSYSpc，ver．2．02软件进行UPGMA聚类分析。结果]10个居群不同变型金樱子的相似性系数在0．09～0．35，聚成A、B2大类。结论]同一变型的金樱子样品并不严格为一类，不同变型金樱子样品之间的遗传变异较大。
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| 关 键 词： | 金樱子 ISSR 遗传变异 |
ISSR Analysis on Rosa laevigata Michx. from Different Variants |
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| WANG Xiao-duo,WANG Xiao-li.ISSR Analysis on Rosa laevigata Michx. from Different Variants[J].Journal of Anhui Agricultural Sciences,2014(17):5425-5426. |
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| Authors: | WANG Xiao-duo WANG Xiao-li |
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| Institution: | et al (Huaxi Agricultural Bureau, Guiyang, Guizhou 550025 ) |
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| Abstract: | Objective ] The heritable variation of 10 was uncovered for different variants of Rosa laevigata Michx. in Guizhou. Method ] By u- sing ISSR molecular marker technology, effective primers were screened from random primers, and were amplified by ISSR. UPGMA cluster analysis was conducted by NTSYSpc, ver. 2.02. Result] The similarity coefficients of the 10 populations were from 0.09 to 0. 35, and divided into 2 groups for A and B. Conclusion] Individuals of the same population did not strictly cluster together and the genetic diversity of individuals in different populations was considerably high. |
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| Keywords: | Rosa laevigata Michx ISSR Heritable variation |
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