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动物细胞培养物中支原体污染的检测
引用本文:赵俊,王兴满,胡勇,陈敬贤,王明丽.动物细胞培养物中支原体污染的检测[J].安徽农业科学,2010,38(3):1151-1153.
作者姓名:赵俊  王兴满  胡勇  陈敬贤  王明丽
作者单位:安徽医科大学微生物学教研室,安徽合肥,230032;安徽医科大学微生物学教研室,安徽合肥,230032;安徽医科大学微生物学教研室,安徽合肥,230032;安徽医科大学微生物学教研室,安徽合肥,230032;安徽医科大学微生物学教研室,安徽合肥,230032
基金项目:安徽省自然科学基金研究重点项目(KJ2008A085);;安徽省科技攻关项目(08010302179);;2008年国家自然科学基金面上项目(30872253)
摘    要:目的]建立检测细胞培养物中支原体快速有效的方法。方法]从6种常见污染细胞的支原体16sRNA中选择2段高度保守的核酸序列作为引物,用PCR和DNA荧光染色法来检测实验室中25种动物细胞株。结果]用DNA荧光染色法检测实验室中25种动物细胞株阳性率为24%,可疑阳性为16%;而用PCR法检测阳性率为36%。结论]PCR法较DNA荧光染色法灵敏度更高、特异性更强,将2种方法结合应用效果更好。

关 键 词:支原体  细胞培养  DNA荧光染色法  聚合酶链反应

Detection of Mgcoplasme Contamination in Animal Cell Culture
ZHAO Jun et al.Detection of Mgcoplasme Contamination in Animal Cell Culture[J].Journal of Anhui Agricultural Sciences,2010,38(3):1151-1153.
Authors:ZHAO Jun
Institution:Teaching and Research Section of Microbiology;Anhui Medical Uriversity;Hefei;Anhui 230032
Abstract:Objective]To establish a rapid and efficient method for detecting the mycoplasma contamination of cell cultures .Method]Two highly conserved nucleotide sequences were chosen as coplasma' primers from 16sRNA in the six common kinds of mycoplasma contaminated cells. PCR and DNA fluorescence staining method were selected to detecte 25 kinds of animals cell lines in laboratory.Result]The positive rate is 24% by DNA fluorescence staining and the suspicious positive is 16%. The positive rate is 36% by PCR.Conclusion]The PCR method has a higher sensitivity and a greater specificity than DNA fluorescence staining.The effect of the combined use them is better.
Keywords:Mycoplasma  Cell Culture  DNA fluorescence staining  Polymerase chain reaction  
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