| 西兰苔籽中硫代葡萄糖苷酶解条件的研究及产物的鉴定 |
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| 引用本文： | 张博超,杨艳婧,张学武.西兰苔籽中硫代葡萄糖苷酶解条件的研究及产物的鉴定[J].安徽农业科学,2011,39(1):97-99. |
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| 作者姓名： | 张博超 杨艳婧 张学武 |
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| 作者单位： | 华南理工大学轻工与食品学院,广东广州,510640;华南理工大学轻工与食品学院,广东广州,510640;华南理工大学轻工与食品学院,广东广州,510640 |
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| 摘 要： | 目的]研究西兰苔籽中硫代葡萄糖苷酶解的影响因素,确定硫苷酶解的最佳条件并分析和鉴定酶解产物。方法]以西兰苔籽粉末为材料,研究不同的温度、pH以及时间对酶水解的影响,并且利用气相色谱-质谱连用技术分析鉴定了酶解产物。结果]酶解时间为8h,水解温度为25℃,pH值为7是最佳酶解条件。利用气象色谱-质谱连用技术分析鉴定出5种化合物,占硫苷酶解产物中挥发性化合物总量的80%。结论]确定了西兰苔籽中硫代葡萄糖苷酶解的最佳条件,并为西兰苔籽的抗癌活性研究提供了一定的理论基础。
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| 关 键 词： | 西兰苔籽 硫代葡萄糖苷 酶解 气相色谱-质谱 |
Study on Enzymatie Hydrolysis Conditions of Glueosinolate in Broccolini Seeds and Identify the Products |
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| ZHANG Bo-chao et al.Study on Enzymatie Hydrolysis Conditions of Glueosinolate in Broccolini Seeds and Identify the Products[J].Journal of Anhui Agricultural Sciences,2011,39(1):97-99. |
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| Authors: | ZHANG Bo-chao |
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| Institution: | ZHANG Bo-chao et al (College of Light Industry and Food Science,South China University of Technology,Guangzhou,Guangdong 510640) |
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| Abstract: | Objective]The aim was to research the impact of factors of enzymatic hydrolysis for glueosinolate in broccolini seeds,determine the optimum conditions of enzymatic hydrolysis and identify the enzymatic hydrolysis products.Methods] Take the broccolini seeds powder as the material,the impact of the different temperatures,pH value and time respectively was analysed and the enzymatic hydrolysis products were identified by GC-MS.Result]The results showed that the optimum enzymatic hydrolysis conditions for th... |
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| Keywords: | Broccolini seeds Glucosinolate Enzymatic hydrolysis GC-MS |
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