洋桔梗离体快繁技术研究

目的]建立洋桔梗高效的快速繁殖体系,为工厂化生产提供可行的操作程序。方法]以国外进口的洋桔梗F1代种子(品种名称为Polestar yellow)无菌苗的叶片为外植体,对不定芽的诱导、单芽增殖、无菌苗生根等进行了研究。结果]结果表明:6-BA和IBA组合对洋桔梗叶片不定芽的诱导效果较6-BA和NAA组合好,叶片最佳的分化培养基为MS+6-BA0.6 mg/L+IBA0.04 mg/L,1个月内正常芽平均分化数为8.3;最佳的单芽增殖培养基为MS+6-BA0.8 mg/L+NAA0.04 mg/L,1个月内正常芽的增殖倍数为7.4;无菌苗在含有IBA0.25 mg/L的1/2 MS培养基上,生根率、平均每苗的根数均最大。结论]该结果为洋桔梗的工厂化生产奠定了基础。

Study on Rapid Propagation Technology of Eustoma grandiflorum in vitro

Objective] This study aimed to establish a feasible manipulation procedure for rapid propagation of a new Eustoma grandiflorum species in a factory.Method] Using the aseptic seedling leaves of a newly imported Eustoma grandiflorum species(Polestar yellow) as explants,induction of the adventitious buds,proliferation of single buds and root regeneration of seedlings were studied.Result] The results showed that,for the induction effect of the adventitious buds from leaves,the combination of 6-BA and IBA was better than that of 6-BA and NAA.The best bud induction medium for Polestar yellow was MS + 6-BA 0.6 mg/L + IBA 0.04 mg/L,and the mean number of the normal induced buds was 8.3 per month.The best proliferation medium was MS + 6-BA 0.8 mg/L + NAA 0.04 mg/L,and the proliferation multiple of the normal buds was 7.4 per month.On 1/2 MS medium with IBA 0.25 mg/L,both the root regeneration rate and the mean root number of the plantlets were highest.Conclulsion] These results established a basis for factory production of the new Eustoma grandiflorum species,Polestar yellow