| BIOLOG自动微生物鉴定系统在水产动物病原菌检测中的应用 |
| |
| 引用本文： | 童桂香,韦信贤,黎小正,吴祥庆,庞燕飞.BIOLOG自动微生物鉴定系统在水产动物病原菌检测中的应用[J].安徽农业科学,2011(13):7846-7848. |
| |
| 作者姓名： | 童桂香 韦信贤 黎小正 吴祥庆 庞燕飞 |
| |
| 作者单位： | 广西渔业病害防治环境监测和质量检验中心; |
| |
| 基金项目： | 广西科技攻关项目(桂科攻0992015-1) |
| |
| 摘 要： | 目的]了解BIOLOG自动微生物鉴定系统对水产动物病原菌鉴定结果的可靠性,为水产动物细菌性疾病的诊断提供参考。方法]选用BIOLOG自动微生物鉴定系统对9株分离自发病水产动物的病原菌进行鉴定,同时采用16S rDNA序列分析对其中的4株进行鉴定,比较2种鉴定结果的符合率。结果]采用自动微生物鉴定系统对9株病原菌进行鉴定均取得良好结果,采用16S rDNA序列分析对4株病原菌进行鉴定的结果与应用自动微生物鉴定系统所得结果完全一致,二者符合率为100%。结论]应用BIOLOG自动微生物鉴定系统对水产动物病原菌进行鉴定具有操作简便、快速、准确等特点,是实验室鉴定水产动物病原菌的一种可行方法。
|
| 关 键 词： | BIOLOG自动微生物鉴定系统 水产动物病原菌 16S rDNA 鉴定 |
Application of BIOLOG Automatic Microbiological Assay Systemin the Detection of Aquatic Animals Pathogen |
| |
| TONG Gui-xiang et al.Application of BIOLOG Automatic Microbiological Assay Systemin the Detection of Aquatic Animals Pathogen[J].Journal of Anhui Agricultural Sciences,2011(13):7846-7848. |
| |
| Authors: | TONG Gui-xiang |
| |
| Institution: | TONG Gui-xiang et al(Center of disease prevention and cure,environmental monitoring and quality monitoring of Guangxi fishery,Nanning,Guangxi 530021) |
| |
| Abstract: | Objective] The aim was to know the reliability of BIOLOG automatic microbiological assay system in the detection of aquatic animals' pathogen,providing references for the diagnosis of aquatic animals' bacteriosis.Method] 9 strains pathogen isolated from diseased aquatic animals were identified by BIOLOG automatic microbiological assay system,while 4 strains of them were identified by 16S rDNA sequencing and phylogenetic analysis,then two identification results of them were compared.Result] The results of... |
| |
| Keywords: | BIOLOG automatic microbiological assay system Aquatic animals pathogen 16S rDNA Identification |
| 本文献已被 CNKI 维普 等数据库收录！ |
