不同稀释液对猪精液4℃保存效果的影响研究

目的]利用改进的BF-5稀释液（命名为naBF5），开发一种4℃液态保存猪精子的方法。方法]把猪精子在LEN、BF5和mBF5这3种稀释液中4℃条件下保存5d，按照保存天数分别检测精子的活力、顶体形态、活率和ATP浓度并进行比较。结果]在4℃条件下保存的1～2d，精子的活率在3种稀释液中没有显著的差异；但是第3天开始，3种稀释液中的精子活率有显著差异，在mBF5中保存的精子活率显著地高于在LEN和BF5中保存的精子。第1～5天，在mBF5和B巧稀释液中保存的精子正常顶体的百分率高于LEN稀释液中保存的精予；并且，在4℃保存5d后，在mBF5稀释液中保存的精子正常顶体的百分率要高于在BF5中保存的精子。在4℃条件下保存的第1～5天，mBF5稀释液中的精子活力百分率高于LEN和BF5稀释液保存的精子活力。在3种稀释液中，保存的第1～5天，精子活力的百分率都持续地降低。保存的第1～5天，mBF5稀释液中保存的精子ATP浓度比LEN和BF5稀释液中保存的高。保存5d之后3种稀释液中精子ATP浓度都迅速下降。结论]在4℃条件下保存5d，与LEN和BF5稀释液相比，保存在mBF5稀释液中的精子表现出较高的活力、正常的顶体百分率、活率和ATP浓度。

Study on the Effects of Different Diluents on the Quality of Boar Sperm Stored at 4 ℃

Objective]This study was designed to develop a method of liquid storage of boar sperm at 4 ℃ using the modified BF5 diluent with BSA and N-acetyl-D-glucosamine (mBF5). Method]Boar sperm were stored in LEN, BF5 and mBF5 diluents at 4 ℃ for 5 days,and sperm motility, acrosome morphology, viability and ATP were examined according to preservation period.Result]The results showed that there were no significant differences in sperm motility among the three diluents from 1 day to 2 days stored at 4 ℃. However, the sperm motility showed significant differences among the three diluents after 3 days stored at 4 ℃. The sperm motility in mBF5 diluent was higher than that in LEN and BF5 diluents after 3 days of storage. The percentage of normal acrosome in mBF5 and BF5 diluents was higher than that in LEN diluent from 1 day to 5 days stored at 4 ℃. However, the percentage of normal acrosome in mBF5 diluent was higher than that in BF5 diluent after 5 days stored at 4 ℃. The percentage of sperm viability in mBF5 diluent was higher than that in LEN and BF5 diluent from 1 day to 5 days stored at 4 ℃. The percentage of sperm viability declined steadily from 1 day to 5 days stored in the three different diluents, respectively. Sperm ATP in mBF5 diluent was higher than that in LEN and BF5 diluents from 1 day to 5 days of storage. Sperm ATP declined rapidly after 5 days of storage in the three different diluents, respectively.Conclusion]It was confirmed that boar sperm could be stored in mBF5 diluent at 4 ℃ for 5 days and showed higher motility, normal acrosome, sperm viability and sperm ATP concentration compared with that stored in LEN and BF5 diluents.