马铃薯离体再生与遗传转化研究进展 |
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引用本文: | 郭阳鑫,佘跃辉,薛红卫,许智宏,卫志明,朱木兰.马铃薯离体再生与遗传转化研究进展[J].安徽农业科学,2016,44(36). |
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作者姓名: | 郭阳鑫 佘跃辉 薛红卫 许智宏 卫志明 朱木兰 |
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作者单位: | 中国科学院上海植物生理生态研究所,上海200032;四川农业大学农学院,四川成都611130;四川农业大学农学院,四川成都,611130;中国科学院上海植物生理生态研究所,上海,200032 |
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基金项目: | 复旦大学遗传工程国家重点实验室开放课题( SKLGE -1410);国家高技术研究发展计划“863”计划(2013AA102704)。 |
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摘 要: | 探讨了以马铃薯茎段、叶片及块茎为起始外植体不同离体再生体系的建立和相关影响因素,分析了基因型、受体外植体类型、菌液浓度、侵染时间、共培养和预培养等对遗传转化效率的影响,为建立马铃薯高效离体再生体系和遗传转化体系提供理论参考。
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关 键 词: | 马铃薯 植物激素 离体再生 遗传转化 |
The Research Progress of in vitro Regeneration and Genetic Transformation System in Potato |
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Abstract: | The establishment of different potato in vitro regeneration systems and related impact factors were discussed with stem ,leaf and tuber as initial explant;and the effects of genotype,type of receptor system,bacterial concentration,infection time,co-culture and pre-culture on genetic transformation efficiency were analyzed, so as to provide important reference for establishment of high efficiency in vitro regeneration and genetic transformation system in potato. |
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Keywords: | Potato Plant hormone Phytohormone regeneration in vitro Genetic transformation |
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