甘薯新品种万薯7号组培快繁技术研究

目的]探索甘薯新品种万薯7号组培快繁技术。方法]剪取万薯7号0．5～1．0cm长的单叶节段，接种到不同激素处理的MS固体培养基，在温度（27±1）℃，光周期16h／d，光强1500～2000lx条件下培养30d。结果]添加生长素IAA或NAA对薯苗生长起促进作用，而细胞分裂素6-BA抑制生长，0．01-0．80mg／L的IAA或NAA与1．00mg／L的6．BA配合使用均抑制万薯7号组培苗生长。结论]添加0．05mg／L的IAA或0．20mg／L的NAA的MS培养基，能显著增加万薯7号培养苗的叶片数和苗高，增殖系数达5倍以上，适用于万薯7号组培快繁。

Research on the Tissue Culture and Rapid Propagation Techniques of a New Sweet-potato Variety Wanshu 7

Objective] The purpose was to explore the technique of tissue culture and rapid propagation techniques of a new sweet-potato variety Wanshu 7. Method] 0.5-1.0 cm segments of single leaf of Wanshu 7 were pruned and inoculated on MS solid media with different hormone, and cultured for 30 days in the fallowing conditions of temperature of (27±1)℃, photoperiod of 16 h/d and light intensity of 1 500-2 000 lx.Result] The results showed that the addition of auxin IAA or NAA could promote the growth of potato seedlings,but the cytokinin 6-BA inhibited the growth. 0.01-0.80 mg/L auxin IAA or NAA and 1.00 mg/L 6-BA could also inhibit the growth of potato seedlings of Wanshu 7.Conclusion] The proliferation coeffeicent reached more than five times. It was the optimum method for the tissue culture and rapid propagation of Wanshu 7.