6种有机磷农药分离及水中残留高效薄层析测定方法研究

目的]比较有机磷农药的分离方法，用高效薄层色谱测定农药在水中残留。方法]采用高效薄层析技术AMD和双槽展缸上行展开方式分离水中辛硫磷、毒死蜱、双硫磷、甲基异柳磷、喹硫磷和三唑磷6种有机磷农药，并对自来水中4种有机磷残留的测定方法进行研究。结果]两种展开方式均能很好分离6种农药。AMD展开Rf值分布范围较窄（相差0.34），双槽展缸上行展开Rf值分布范围较宽（相差0.76）；AMD多步展开检测灵敏度较双槽展缸上行展开低。采用双槽展缸上行展开薄层色谱法检测水中有机磷农药残留，添加回收率在86.05%-109.80%之间，添加回收率、变异系数均满足农药残留分析的要求。结论]该研究中，双槽展缸上行展开表现出灵敏度较高、操作简便、分析速度快的特点。

Study on Separation of 6 Kinds of Organophosphorous Pesticides and Determination of Their Residues in Water by HPTLC

Objective] The aim was to compare the separating methods of 6 organophosphorous pesticides and determine their residues in water by HPTLC.Method] 6 kinds of organophosphorous pesticides of phoxim,chlorpyrifos,temephos,isofenphos-methyl,quinalphos and triazophos in water were separated by AMD and ascending development method using two-trough chamber,and the determination method of the residues of the 4 kinds of organophosphorous pesticides from water were studied.Result] 2 developing methods all could separate the 6 kinds of organophosphorous pesticides better.The distribution range of Rf value of AMD developing method was narrower(difference was 0.34) and that of ascending development method using two-trough chamber was wider(difference was 0.76).The sensitivity determined by AMD multi-step expansion method was lower than that by ascending development method using two-trough chamber.Using ascending development in two-trough chamber-TLC method determining organophosphorous pesticide residue,the fortified recovery was between 86.05% and 109.80%,then the fortified recovery and variation coefficient all could meet the demand for pesticide residue analysis.Conclusion]In this study,the sensitivity of ascending development using two-trough chamber was higher,and its operation was convenient and analysis speed was rapid.