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黄酮类化合物的提取·分离纯化和含量测定方法的研究进展
引用本文:郭雪峰,岳永德.黄酮类化合物的提取·分离纯化和含量测定方法的研究进展[J].安徽农业科学,2007,35(26):8083-8086.
作者姓名:郭雪峰  岳永德
作者单位:国际竹藤网络中心,国家林业局竹藤科学与技术重点开放实验室,北京,100102
基金项目:国家林业局“948”项目资助(98006),国家“十一五”科技支撑项目(2006BAD24B0802)。
摘    要:综述了黄酮类化合物提取方法(溶剂法、微波法、酶解法、超临界流体萃取法、双水相萃取法、超声波提取法),分离纯化方法(柱层析法、薄层层析法、高效液相色谱法、纸层析法、超临界流体分离法、大孔树脂吸附法、液滴逆流层析法、梯度pH值萃取法、金属试剂络合沉淀法、膜分离法、活性炭吸附法),黄酮含量测定方法(分光光度法、高效液相色谱法、毛细管电泳法、超临界流体色谱法、薄层扫描法),为黄酮类化合物研究工作者提供参考。

关 键 词:黄酮类化合物  提取  分离  纯化  测定
文章编号:0517-6611(2007)26-08083-04
修稿时间:2007-05-15

Research Advances in Extraction, Isolation, Purification and Content Determination of Methods Flavonoids
GUO Xue-feng et al.Research Advances in Extraction, Isolation, Purification and Content Determination of Methods Flavonoids[J].Journal of Anhui Agricultural Sciences,2007,35(26):8083-8086.
Authors:GUO Xue-feng
Institution:International Centre for Bamboo and Rattan;SFA Key Open Laboratory on Bamboo and Rattan Science and Technology;Beijing 100102
Abstract:Extraction methods for flavonoids (solvent method, microwave method, enzyme decomposition method, supercritical fluid extraction method, aqueous two phase extraction and ultrasonic extraction method) were summarized, as well as isolation-purification method for flavonoids (column chromatography method, thin layer chromatography method, high-performance liquid chromatography method, paper chromatography method, supercritical fluid chromatography method,macroporous resin adsorption method, high-speed counter current chromatography method, pH grads extraction method, complexation precipitate method, film filtration method and active carbon adsorption method) and determination methods for flavonoids content (spectrophotometric method, high-performance liquid chromatography method, capillary electrophoreses method, supercritical fluid chromatography method, thin layer chromatography method). All these provided systemic reference for researchers studied on flavonoids.
Keywords:Flavonoids  Extraction  Isolation  Purification  Determination
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