翠菊离体培养及再生体系的建立

目的]建立翠菊离体快速繁殖及再生体系。方法]以无菌播种获得的翠菊试管苗为外植体，以MS为基本培养基，添加不同浓度的6-BA、KT、NAA，对翠菊试管苗进行扩繁及生根培养试验，研究不同生长调节剂对翠菊继代培养和生根培养的影响，筛选翠菊快繁及再生的最佳培养基。结果]6-BA浓度对愈伤组织的诱导有显著影响，随着6-BA浓度增高，诱导率显著增高；KT对愈伤组织的诱导率最低。MS＋6-BA 0.5mg/L＋NAA 0.1mg／L是翠菊离体快繁的适宜培养基，分生数为5.67±0.82，愈伤诱导率为（53.33±0.09）％；MS＋6-BA 2.0mg／L＋NAA 0.2mg,／L为最佳的再生体系建立培养基，分生数为6.33±1.03，愈伤诱导率为（83.70±0.04）％。在MS＋NAA0.2mg／L培养基中，试管苗生根率可达98.0％以上，炼苗移栽成活率可达90.0％以上。结论]该试验建立了翠菊离体快速繁殖及再生体系。

In Vitro Culture and Establishment of Regeneration System of Callistephus chinensis

Objective] The study aimed to establish the rapid propagation in vitro and regeneration system of Callistephus chinensis.Method] With test-tube plantlets of C.chinensis through aseptic seeding as explants,MS basic medium was used as the media by adding different concn.of 6-BA,KT,NAA,the expanding propagation and rooting culture test were carried out on C.chinensis test-tube plantlets so as to research the effect of different growth regulator on subculture and rooting culture of C.chinensis test-tube plant...