| 原生质体融合法选育酵母菌株酿造低甲醇高总酯蒸馏酒 |
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| 引用本文： | 林小江,周世水. 原生质体融合法选育酵母菌株酿造低甲醇高总酯蒸馏酒[J]. 安徽农业科学, 2014, 0(13): 4050+4054-4050,4054 |
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| 作者姓名： | 林小江 周世水 |
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| 作者单位： | 华南理工大学生物科学与工程学院; |
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| 基金项目： | 广东省教育部学研结合项目(2011B090400496) |
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| 摘 要： | [目的]选育酿造低甲醇高总酯蒸馏酒的酿酒酵母菌株.[方法]利用原生质体融合技术,对低甲醇酿酒酵母与高总酯酿酒酵母进行融合.[结果]选育出的酵母菌种酿造蒸馏酒中的相对甲醇含量为145.31 mg/L,比初始低甲醇菌株降低了22.8％,总酯含量为0.79g／L,增加了83.7％.[结论]原生质体融合法成功选育出低甲醇高总酯的酿酒酵母菌株.
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| 关 键 词： | 原生质体融合 蒸馏酒 甲醇 总酯 |
Brewing Distilled Liquor of Low Methanol and High Total Esters with Saccharomyces cerevisiae Bred by Protoplast Fusion |
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| LIN Xiao-jiang;ZHOU Shi-shui. Brewing Distilled Liquor of Low Methanol and High Total Esters with Saccharomyces cerevisiae Bred by Protoplast Fusion[J]. Journal of Anhui Agricultural Sciences, 2014, 0(13): 4050+4054-4050,4054 |
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| Authors: | LIN Xiao-jiang ZHOU Shi-shui |
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| Affiliation: | LIN Xiao-jiang;ZHOU Shi-shui;College of Biological Science and Engineering,South China University of Technology; |
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| Abstract: | [Objective] Screening Saccharomyces cerevisiae of brewing distilled liquor with low methanol and high total esters.[Method] Technology of protoplast fusion was used to fusing Saccharomyces cerevisiae of low methanol and Saccharomyces cerevisiae of high total esters.[Result]The methanol was 145.31 mg/L in the distilled liquor fermented by protoplast fusion,decreasing 22.8％ than strain of low methanol.The total ester was 0.79 g/L,increasing 83.7％.[Conclusion] The Saccharomyces cerevisiae of low methanol and high total esters was obtained by technology of protoplast fusion. |
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| Keywords: | Protoplast fusion Distilled liquor Methanol Total esters |
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