翅果油树总生物碱的抑菌作用

目的]为将翅果油树总生物碱作为植物源农药的可行性提供理论依据。方法]用超声辅助法从翅果油树叶片中提取总生物碱,经系统分离获得5种生物碱组分,并测试各生物碱组分的抑菌活性。结果]5种翅果油树生物碱组分对大肠杆菌革兰氏阴性菌的抑菌作用不明显;对枯草芽孢杆菌、金黄色葡萄球菌、革兰氏阳性菌以及苹果树腐烂病菌、棉花黄萎病菌的抑菌作用明显,其中非酚性叔胺性生物碱抑菌作用最强,对枯草芽孢杆菌、金黄色葡萄球菌的最低抑菌浓度分别为0.4和0.8 mg/ml;当非酚性叔胺性生物碱实际作用浓度为3.2 mg/ml时,对苹果树腐烂病菌和棉花黄萎病菌的抑制率分别达到72.7%和93.4%。翅果油树生物碱具有很强的热稳定性,高温处理不会降低其抑菌活性;酸化处理会降低其抑菌活性,而在经过酸化处理的抑菌液中,生物碱在中性条件下抑菌效果最强。结论]翅果油树中提取的总生物碱有希望开发成广谱性植物源杀菌剂。

Study on Antibacterial Activity of Alkaloids from Elaeagnus mollis′s Diels

Objective]The study aimed at providing theoretical evidence for the feasible utilization of alkaloids from Elaeagnus mollis Diels as botanical pesticide.Method]Ultrasonic-assisted extraction was used to extract alkaloids,and five kinds of alkaloids were obtained,the antibacterial activity of which was determined.Result]The antibacterial activity of 5 alkaloids on E.coli and gram negative bacteria were not strong,while than on B.subtilis,S.aureus,gram positive bacteria,V.ceratosperma and Verticillium dahl...