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| 2种方法提取日本沼虾基因组DNA | |
| 引用本文： | 李艳和,陈宏权,汪留全,梁忠,张同燕,甘小伟,胡王.2种方法提取日本沼虾基因组DNA[J].安徽农业科学,2006,34(21):5487-5488. |
| 作者姓名： | 李艳和 陈宏权 汪留全 梁忠 张同燕 甘小伟 胡王 |
| 作者单位： | 1. 安徽农业大学,安徽合肥,230036;安徽省农业科学院水产研究所,安徽合肥,230036 2. 安徽农业大学,安徽合肥,230036 3. 安徽省农业科学院水产研究所,安徽合肥,230036 |
| 基金项目： | 安徽省财政厅成果推广项目. |
| 摘 要： | 以日本沼虾的尾部肌肉为材料,采用苯酚-氯仿法和氯化钠法提取日本沼虾基因组DNA,并对2种方法提取的结果进行了紫外分光光度、琼脂糖凝胶电泳、PCR扩增等方法的鉴定。结果表明:2种方法提取的日本沼虾基因组DNA浓度分别为3 165.8和396.5ng/μl,OD260/OD280比值分别为1.76和1.63,均能满足分子生物学研究的需要。 |
| 关 键 词： | 日本沼虾 基因组DNA 提取方法 |
| 文章编号： | 0517-6611（2006）21-5487-02 |
| 收稿时间： | 09 18 2006 12:00AM |
| 修稿时间： | 2006-09-18 |
DNA Extraction Methods for Macrobrachium nipponense DNA |
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| LI Yan-he, et al.DNA Extraction Methods for Macrobrachium nipponense DNA[J].Journal of Anhui Agricultural Sciences,2006,34(21):5487-5488. | |
| Authors: | LI Yan-he |
| Institution: | Anhui Agricultural University,Hefei,Anhui 230036 |
| Abstract: | Genomic DNA of Macrobrachium nipponense was extracted by means of two different methods of trichlorophenol-trichloromethane and sodium Chloride.The extraction DNA quality of the two methods was detected by means of Ultraviolet Spectrophtometry,agarose gel and PCR amplification.The results showed that the DNA sample obtained with the two methods got the highest purity(OD_(260)/OD_(280)=1.76,1.63) and concentration(3?165.8,396.5?ng/μl).The result of PCR indicated that the quality of the DNA isolated with the two methods could meet the requirements of Macrobrachium nipponense molecular biology manipulation. |
| Keywords: | Macrobrachium nipponense Genomic DNA Extraction method |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |