| 端粒酶RNA亚基的shRNA对细胞端粒酶活性影响的研究 |
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| 引用本文： | 张哲,明宇,于聪,张玉静.端粒酶RNA亚基的shRNA对细胞端粒酶活性影响的研究[J].安徽农业科学,2009,37(1):147-149. |
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| 作者姓名： | 张哲 明宇 于聪 张玉静 |
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| 作者单位： | 吉林大学畜牧兽医学院,吉林长春,130062;吉林大学人兽共患病研究所,吉林长春,130062;中国科学院长春应用化学研究所,吉林长春,130022 |
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| 摘 要： | 目的]探讨端粒酶RNA亚基（chTR）的短发RNA（shRNA）shRNA对MDCC-MSB1细胞端粒酶活性的影响。方法]设计合成chTRshRNA并构建表达载体,转染MDCC-MSB1细胞,应用改良TRAP法检测端粒酶活性。结果]与对照组相比,转染24 h后,各组端粒酶活性变化不明显;转染48 h后,端粒酶活性均显著地下降,且针对模板区设计的干扰载体pSi-chTR-sh1转染后抑制效果最明显。结论]chTRshRNA表达载体能够有效地抑制MDCC-MSB1细胞的端粒酶活性。
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| 关 键 词： | 端粒酶 端粒酶RNA shRNA |
Study on the Effect of shRNA of chTR on Telomerase Activity of Cell |
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| ZHANG Zhe et al.Study on the Effect of shRNA of chTR on Telomerase Activity of Cell[J].Journal of Anhui Agricultural Sciences,2009,37(1):147-149. |
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| Authors: | ZHANG Zhe |
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| Institution: | ZHANG Zhe et al (College of Animal Science , Veterinary Medicine,Jilin University,Changchun,Jilin 130062) |
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| Abstract: | Objective] The aim of this study was to investigate the inhibitory effects of shRNA of chTR on telomerase activity in MDCC-MSB1 cells.Method] The artificially designed and synthesized short hairpin RNA(shRNA) of chicken telomerase RNA(chTR) was used to construct the expression vector that was subsequently transfected to MDCC-MSB1 cells,then the telomerase activity of these cells was measured by improved TRAP method.Result] Compared to the CK group,the telomerase activities in the treatment cells showed n... |
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| Keywords: | Telomerase Telomerase RNA shRNA |
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