朝鲜蓟外植体诱导研究

目的]筛选诱导朝鲜蓟愈伤组织的最佳培养基和培养条件。方法]用朝鲜蓟的种子、根、叶柄、第三展开叶、第二展开叶、第一展开叶、心叶为外植体,以MS培养基为基本培养基,研究不同激素种类、不同浓度组合的4种培养基对朝鲜蓟愈伤组织的诱导及分化的影响以及不同外植体形成愈伤组织的能力。结果]按朝鲜蓟愈伤组织诱导率大小,4种培养基组合的排序为:MS+6-BA 2.0 mg/L+NAA 0.2 mg/L>MS+6-BA 2.0 mg/L+NAA 0.5 mg/L>MS+6-BA 2.0 mg/L+NAA 1.0 mg/L>MS+6-BA 0.5 mg/L+NAA 2.5mg/L;外植体的排序为:第一展开叶>心叶>第二展开叶>叶柄>第三展开叶>根>种子。综合分析表明,诱导朝鲜蓟愈伤组织的最佳条件为:以第一展开叶和心叶为外植体,在MS+6-BA 2.0 mg/L+2,4-D 0.5 mg/L培养基上,诱导率可达90.5%。结论]该研究为朝鲜蓟的组织培养和快速繁殖提供技术依据。

Study on the Induction of Explants of Artichoke

Objective] The study aimed to screen the optimum medium and the cultural condition for inducting the artichoke callus.Method] With the seeds,root,leafstalk,third unrolled leaf,second unrolled leaf,first unrolled leaf and heart leaf as the explants and MS medium as the basic medium,the 4 combinations of media with different phytohormone kinds and concn.on the induction and differentiation of artichoke callus and the ability of forming callus by different explants.Result] The induction rate of artichoke callus on 4 combinations of media from big to small was follows: MS 6-BA 2.0 mg/L NAA 0.2 mg/L > MS 6-BA 2.0 mg/L NAA 0.5 mg/L> MS 6-BA 2.0 mg/L NAA 1.0 mg/L > MS 6-BA 0.5 mg/L NAA 2.5 mg/L and that of different explants in order was follows: first unrolled leaf > heart leaf > second unrolled leaf > leafstalk > third unrolled leaf > root > seed.The comprehensive analysis showed that the optimum condition for inducing the artichoke callus was follows: when first unrolled leaf and heart leaf as the explant were cultured on the medium of MS 6-BA 2.0 mg/L 2,4-D 0.5 mg/L,the induction of artichoke callus could reach 90.5%.Conclusion] The study provided the technique basis for tissue culture and rape propagation of artichoke.