| 一株H9N2亚型禽流感病毒全基因组序列的遗传变异分析 |
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| 引用本文： | 于博,章振华,姜北宇,钱爱东,李林,景小冬,张建伟.一株H9N2亚型禽流感病毒全基因组序列的遗传变异分析[J].安徽农业科学,2010,38(1):507-509,526. |
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| 作者姓名： | 于博 章振华 姜北宇 钱爱东 李林 景小冬 张建伟 |
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| 作者单位： | 吉林农业大学动物科技学院,吉林长春,131200;北京市农林科学院畜牧兽医研究所,北京,100097;北京市农林科学院畜牧兽医研究所,北京,100097;吉林农业大学动物科技学院,吉林长春,131200 |
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| 基金项目： | 国家973资助项目子课题(2005CB523001) |
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| 摘 要： | 目的]了解H9N2亚型禽流感病毒分离毒株A／Chicken／Hebei／WD／98（简称WD98）与其他参考毒株的遗传变异关系。方法]采用RT—PCR技术扩增了WD98分离株8个基因片段的全基因序列，并将其与7个参考毒株进行同源性比较及遗传进化分析。结果]分离株WD98与7个参考毒株全基因组同源性为91．1％～95．8％，与A／duck／HongKong／Y280／97的全基因组核苷酸同源性最高（95．8％），而与A／chicken／Pakistan／2／99同源性最低（91．1％）。WD98株的HA基因裂解位点为R—S-S—R↓G，其第226位受体结合住点为Q。结论]禽流感病毒WD98毒株为低致病性禽流感病毒，不易感染人，与A／duck／HongKong／Y280／97亲缘关系最近，同属于欧亚种系A／Chicken／Beijing／1／94亚系。而在这同一亚系中，WD98株与A／Chicken／Beijing／1／94株的亲缘关系最远。
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| 关 键 词： | 禽流感病毒 H9N2 全基因组 遗传变异 |
Genetic Variation Analysis on the Whole Genomic Sequence of a H9N2 Subtype Avian Influenza Virus Isolate |
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| YU Bo et al.Genetic Variation Analysis on the Whole Genomic Sequence of a H9N2 Subtype Avian Influenza Virus Isolate[J].Journal of Anhui Agricultural Sciences,2010,38(1):507-509,526. |
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| Authors: | YU Bo |
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| Institution: | College of Animal Science and Technology;Jilin Agricultural University;Changchun;Jilin 131200 |
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| Abstract: | Objective] To understand the genetic variation characters of the H9N2 subtype avian influenza virus isolate(A/Chicken/Hebei/WD/98,WD98 for short) by comparing with other reference strains.Method] The eight complete genes were amplified by RT-PCR and sequenced.The homology and genetic evolution relation were analyzed between these sequences and that of the seven reference strains.Result] The whole genomic sequence of the WD98 strain was 91.1%-95.8% homologous to that of the seven reference strains.This is... |
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| Keywords: | Avian influenza virus H9N2 subtype Genomic sequence Genetic variation |
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