NaCl胁迫对大豆种子萌发及α-淀粉酶活性的影响

目的]探讨盐胁迫时大豆种子萌发及α-淀粉酶活性变化的影响。方法]用不同浓度的NaCl盐溶液处理大豆种子，对大豆种子的发芽率、发芽势、发芽指数和大豆种子萌发过程中淀粉酶活性进行测定分析。结果]结果表明，NaCl溶液浓度低于60mmol／L时，对大豆种子的发芽势、发芽指数的影响较小，而NaCl溶液浓度高于90mmol／L时，对大豆种子的发芽率、发芽势等抑制作用显著增强；发芽第3天大豆的α-淀粉酶活力最高，随着NaCl溶液浓度的增加，α-淀粉酶的活力呈下降趋势。结论]该研究为大豆种子萌发条件的选择提供科学依据。

Effects of NaCl Stress on Seeds Germination and α-amylase of Soybean

Objective]The purpose of research was to discuss the effects of NaCl stress on seeds germination and α-amylase of soybean.Method] NaCl solutions with defferent concentration were used for saline stress to measure and analyze the seed germination percentage,vigor,index and amylase of soybean by saturating the seeds.Result]The results showed that the germination rate,germination index of the seeds treated with NaCl concentration(≤60 mmol/L) was not significantly comparing with control.However,the germination index and germination rate of those seeds which were treated with NaCl solutions at higher concentration(≥90 mmol/L) was swelled evidently.The activiy of amylase was the highest in the third day and dropped along with the increase of NaCl concentration.Conclusion]This study will provide the scientific basis for choice of soybean seeds germination conditions.