种子带菌作为玉米弯孢菌叶斑病初侵染源的研究

目的]对种子带菌作为玉米弯孢菌叶斑病的初侵染源进行研究,为生产上防治该病提供理论依据。方法]采用玻片萌发法和组织分离法测定种子外部带菌、内部带菌的存活力;采用沙培法在室内检测种子带菌对幼苗的侵染;应用田间培养法测定种子带菌对玉米的侵染。结果]玉米种子所带弯孢菌孢子和菌丝可以越冬成活,种子内部带菌影响种子萌发,种子外部带菌不影响种子萌发;种子内外带菌均能侵染玉米幼苗的胚根和胚芽。结论]玉米种子带弯孢菌孢子和菌丝可以越冬并侵染幼苗,使幼苗长势衰弱。

Study On the Bacterial Carrying of Seeds as the Primary Infection Sources of Curvularia Leaf Spot of Maize

Objective]The aim was to study the bacterial carrying of seeds as the primary infection sources of curvularia leaf spot of maize,and to provide the theoretical basis for the control of the disease in production.Method]Through slide germination and seed tissue isolation method,the viabilities of seeds with internal and external bacterial carrying were detected;the infection of bacterial carrying of seeds on the young seedling was detected indoors by sand culture method;and the infection of bacterial carrying of seeds on maize was detected using field cultivation method.Result]The conidia of curvularia and mycelia carried by maize seeds could survive through the winter.Internal bacterial carrying of seed affected the germination of seeds,but external bacterial carrying did not affect the germination;internal and external bacterial carrying of seeds could infect the radicle and gemmule of maize seedling.Conclusion]The conidia of curvularia and mycelia carried by maize seeds could survive through the winter and infect the seedling,which would make the seedlings have weak growth.