| 广州桑树矮缩病植原体secE基因序列分析与蛋白结构预测 |
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| 引用本文： | 胡小华,张丽君,罗焕亮,张毅.广州桑树矮缩病植原体secE基因序列分析与蛋白结构预测[J].安徽农业科学,2011,39(4):1980-1983,2004. |
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| 作者姓名： | 胡小华 张丽君 罗焕亮 张毅 |
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| 作者单位： | 胡小华(华南理工大学生物科学与工程学院,广东广州,510006;深圳职业技术学院化生院,广东深圳,518055);张丽君(深圳职业技术学院化生院,广东深圳,518055);罗焕亮(深圳出入境检验检疫局,广东深圳,518045);张毅(华南理工大学生物科学与工程学院,广东广州,510006) |
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| 基金项目： | 深圳市基础研究计划项目 |
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| 摘 要： | 目的]预测广州桑树矮缩病植原体secE蛋白的抗原性。方法]应用PCR技术从典型矮缩症状的桑树植株总DNA中扩增到约400 bp的特异片段,将此片段克隆后与GeneBank中已报道的5种植原体的secE基因进行序列同源性分析,并对克隆基因所编码的蛋白进行理化性质、二级结构、跨膜区以及前导信号序列分析。结果]该片段长411 bp,编码136个氨基酸的蛋白。桑树矮缩植原体与洋葱黄化植原体同源性最高,核苷酸和编码的氨基酸序列同源率分别为100%和99%。secE蛋白理化性质预测其理论等电点为9.33;二级结构主要为螺旋,其次为折叠和无规则卷曲,无转角;有3个明显的疏水区,易形成跨膜螺旋;有4个明显的抗原区域。结论]该secE蛋白具有良好的抗原性。
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| 关 键 词： | 广州桑树矮缩病 植原体 SecE基因 同源性 蛋白结构 |
Analysis of secE Gene Sequence from Phytoplasma Associated with Mulberry Dwarf Disease in Guangzhou and Prediction of its Protein Structure |
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| HU Xiao-hua et al.Analysis of secE Gene Sequence from Phytoplasma Associated with Mulberry Dwarf Disease in Guangzhou and Prediction of its Protein Structure[J].Journal of Anhui Agricultural Sciences,2011,39(4):1980-1983,2004. |
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| Authors: | HU Xiao-hua |
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| Institution: | HU Xiao-hua et al(Department of Bioscience & Bioengineering,South China University of Technology,Guangzhou,Guangdong 510006) |
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| Abstract: | Objective]The aim was to predict the antigenicity of secE protein from phytoplasma associated with mulberry dwarf disease in Guangzhou.Method]A fragment about 400bp was amplified by polymerase chain reaction from the total DNA of infected mulberry plants with typical dwarf symptom.The fragment was cloned followed by nucleotide acid sequence analysis with other 5 phytoplasmas of GenBank available.The analysis of physiochemical property,secondary structure,transmembrane domains and preamble signal sequence ... |
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| Keywords: | MD-guangzhou Phytoplasma secE gene Homology Structure of protein |
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