一种适用于动物与植物总DNA提取的方法——改良CTAB法

目的]介绍一种简单、高效且能用于提取动物与植物的总DNA的方法。方法]采用改良CTAB法,从24个花生品种嫩叶及小白鼠的肝、肺和肾中提取DNA,进行琼脂糖电泳和蛋白核酸分析仪检测及PCR扩增检验。结果]所提取DNA电泳条带清晰,整齐均匀,OD260/OD280值介于1.77~1.83,用于PCR扩增获得理想效果。结论]该研究介绍的改良CTAB法提取动物和植物的总DNA,满足开展PCR扩增的要求。

A Method Suitable for Extracting Genomic DNA from Animal and Plant——Modified CTAB Method

Objective] The study aimed to introduce a rapid and effective method that is suitable for extracting genomic DNA from animal and plant.Method] The genomic DNAs were extracted from tender leaves of 24 peanut cultivars and from the liver,lung and kidney of white mouse through the specifically modified CTAB method.The DNAs were run on agarose gel,next detected by DNA/Protein analyzer.Finally PCR amplification was conducted to detect the quality of DNAs extracted using the modified CTAB method.Result] The clear and orderly bands were observed in gel detection,and the values of OD260/OD280 for DNAs extracted via modified CTAB method were between 1.77-1.83.The DNAs performed well in PCR amplification.Conclusion] The DNAs extracted by modified CTAB method could satisfy the requirement of PCR amplification.