蚊净香草的离体快繁工艺

目的]研究蚊净香草试管繁殖的诱导、分化、继代、生根、移栽等过程,筛选优良试管苗。方法]以蚊净香草的侧芽、展开幼叶为外植体,以MS为基本培养基,加不同浓度的6-BA、IAA,进行外植体的启动培养。结果]侧芽适宜的诱导培养基为6-BA 0.2 mg/L+IAA 0.1 mg/L;叶片和叶柄的最佳诱导分化培养基为6-BA 0.5~1.0 mg/L+IAA 0.25~0.50 mg/L;快速繁殖培养基以6-BA 0.2 mg/L+IAA 0.1 mg/L最好;生根培养基以1/2MS+NAA0.2 mg/L效果较好;适宜的移栽基质为草炭∶蛭石∶珍珠岩=3∶2∶1。结论]移栽时用保护性杀菌剂多菌灵等防止杂菌的滋生,可以有效提高移栽成活率。

Rapid Propagation Technique in vitro of Pelargonium odoratissmum

Objective] The research aimed to study the tube propagation process of induction,differentiation,subculture,rooting,transplanting of P.odoratissmum,and to screen fine tube seedlings.Method]The lateral bud and spread young leaf of Pelargonium odoratissmum was used for explant,MS for basic medium,adding different contents 6-BA,TAA.Then the initiation culture of explant was carried out.Result] The superior induction medium of lateral bud was 6-BA 0.2 mg/L+IAA 0.1 mg/L.The superior inductive differentiation medium of leaf and petiole was 6-BA 0.5～1.0 mg/L +IAA 0.25～0.50 mg/L.The superior rapid propagation medium was 6-BA 0.2 mg/L +IAA 0.1 mg/L.The superior rooting medium was 1/2MS+NAA 0.2 mg/L.The suitable ratio of transplanting medium for peat∶vermiculite∶perlite was 3∶ 2∶1.Conclusion] Protective fungicide such as carbendazim was used to inhabit mixed bacterium.And the rate of transplanting could be improved at the same time.