高效液相色谱法快速检测食品中苏丹红的含量

目的]建立快速检测食品中苏丹红（苏丹红I、Ⅱ、Ⅲ、IV）含量的高效液相色谱法，简化样品的前处理，降低成本，缩短仪器检测时间。方法]选用Athena C18-XDB色谱柱（4．6mm×150．0mm，3．5um），以乙腈水溶液和乙腈丙酮溶液为流动相进行等度洗脱，通过二极管阵列检测器检测食品中苏丹红。结果]该方法检测线性良好，相关系数R≥0．9991，检出限为0．01mg／L，相对标准偏差（n=10）为0．9％～2．0％，回收率为90％～96％。结论]该法具有较高的准确度和精密度，适用于食品中苏丹红含量的测定。

High Performance Liquid Chromatographic Method for Rapid Determination of Sudan in Food

Objective] A analytical method was established to determine Sudan （Sudan I, II, III , IV） content in food by high performance liquid chromatography （HPLC） with simplified sample pre-treatment, reduced cost and shorten instrument detection time. Method] The Sudan were separated on an Athena C,s-XDB column （4.6 mm × 150.0 mm, 3.5 um） by isocratic elution using acetonitrile water solution and acetonitrile acetone solution as mobile pbase, and analyzed with a diode-array detector. Result] The method showed a good linear relationship with correlation coefficient of more than 0. 999 1. The limit of detection （LOD） was 0. 01 mg/L, the relative standard deviation （ n = 10） was between 0.9% -2.0% , and the recovery rate was between 90% -96%. Conclusion] The method has a high accuracy and precision, which is suitable for analyzing the Sudan content in food.