川芎的组织培养技术研究

探索川芎的不同组培条件,优化其诱导和分化培养基。以川芎根、茎段和叶片作外植体进行组织培养,获得了大量的组培苗,建立了相应的植株再生系统。川芎根诱导愈伤组织的最佳培养基为MS+6-BA 0.8 mg/L+NAA 1.2 mg/L;茎段及叶片诱导愈伤组织的最佳培养基均为MS+6-BA 0.5 mg/L+NAA 1.5 mg/L;根愈伤组织分化不定芽的最佳培养基为MS+6-BA 2.2 mg/L+IAA 0.3 mg/L;茎段及叶片愈伤组织分化不定芽的最佳培养基为MS+KT 2.0 mg/L+IAA 0.5 mg/L。根外植体在愈伤组织、分化不定芽和生根方面的诱导率分别为84%、86%和87%;茎段和叶片愈伤组织的诱导率达到92%和96%,其不定芽分化率为98%,生根率为93%。经炼苗后,获得的组培苗的移栽成活率达98%。提出了优化激素搭配的培养基,得到了高效的诱导率、分化率和生根率。

Study on the Tissue Culture Technique of Ligusticum chuanxiong Hort.

The study aimed to explore different tissue culture conditions of L.chuanxiong and optimize its induction and differentiation medium.In tissue culture with the root,stem segments and leaf of L.chuanxiong as explants,a lot of plantlets were obtained and the corresponding plant regeneration system was established.The optimum medium for inducing callus with L.chuanxiong root was MS 6-BA 0.8 mg/L NAA 1.2 mg/L and that with L.chuanxiong stem segments and leaf was MS 6-BA 0.5 mg/L NAA 1.5 mg/L.The optimum medium for differentiating adventitious buds from root callus was MS 6-BA 2.2 mg/L IAA 0.3 mg/L and that form stem segments and leaf was MS KT 2.0 mg/L IAA 0.5 mg/L.The callus,differentiating adventitious bud and rooting induction of root explants were 84%,86% and 87% respectively.The callus induction rates of stem segments and leaf were 92% and 96% and their differentiation rate and rooting rate of adventitious bud were 98% and 93%.The transplanting survival rate of obtained plantlets was 98% seedling after-training.The medium with optimizing hormone combination was put forward and the high-effective induction rate,differentiation rate and rooting rate were obtained.