基于胶体金免疫层析法快速筛查水产品中的药物残留

目的]应用现代检测技术,对水产品中药物残留实施监控,推动水产品市场准入制度和基地准出制度的实施.方法]运用胶体金免疫层析法对采集的水产样品中可能残留的违禁药物进行快速筛查.结果]试验显示,此方法检测限能达到：氯霉素≤0.5 μg/kg、孔雀石绿≤1.0 μg/kg、硝基呋喃代谢物≤1.0μ/kg、氟喹诺酮≤10 μg/kg,在一定程度上满足了基层对水产品质量安全监管需要,大大减少了基层对水产品安全检测的盲区.结论]胶体金免疫层析法（GICA）是一种新型快速免疫检测技术,具有特异性强、操作简便快捷、检测成本低等优点,研究可为水产品中的违禁药物残留监控提供技术支持.

Rapid Screening of Drug Residues in Aquatic Products Based on Colloidal Gold Immunochromatographic Assay

Objective] To monitor drug residues in aquatic products by using modern detection technique and promote operation of aquatic product market access system.Method] GICA was adopted for rapid screening of illicit drug residue in collected aquatic products.Result]The results showed that the method detection limit can reach：chloramphenicol ≤ 0.5 μg/kg,malachite green ≤ 1.0 μg/kg,metabolites of nitrofuran ≤ 1.0 μg/kg,fluoroquinolone ≤ 10 μg/kg,which meet the demands on aquatic product quality safety monitoring and greatly reduce the blind zone in aquatic product safety detection.Conclusion] GICA is a new rapid immune detection technology with advantages of strong specificity,convenient operation,low detection cost,which provides technical support for monitoring illicit drug residue in aquatic product.