大豆异黄酮的提取纯化及抗氧化性研究

目的]对大豆异黄酮提取纯化的最佳工艺条件及其抗氧化活性进行研究.方法]通过单因素试验和L9（34）正交试验,确定提取大豆异黄酮的最佳工艺条件,应用D101大孔树脂技术对提取液进行进一步分离纯化,得出最佳纯化条件,并对纯化得到的染料木苷和大豆苷进行抗氧化活性研究.结果]试验得出,提取大豆异黄酮的最佳工艺条件为乙醇浓度70％,料液比1∶15 g/ml,提取时间为3h,提取温度为60℃,最高得率达9.18％;纯化最佳条件为：上柱静态吸附时间5h,洗脱时间30 min,80％乙醇作为洗脱剂,洗脱流速为lml/min,并分离纯化得到染料木苷和大豆苷;抗氧化活性研究表明,染料木苷、大豆苷和大豆总黄酮对超氧阴离子自由基和羟自由基均具有清除作用.结论]研究对大豆保健食品开发和天然药物研制具有重要意义.

Extraction,Purification Technology and Antioxidation Activities of Soybean Isoflavones

Objective]To study the optimum technique conditions and antioxidation activities of extraction and purification of soybean isoflavones.Method]On the basis of single element experiments and L9 （34） orthogonal experiments,the optimum technique conditions for extraction of soybean isoflavone were determined.D101 macroporous resin technology was applied to further purify the liquids.Result]The optimum extraction conditions were obtained：ethanol concentration 70％,soybean to solvent ratio 1∶15 g/ml,extraction time 3 h,temperature 60 ℃.The extraction yield could be up to 9.18％.Purification process of soy isoflavones in soybean by using D101 macroporous resin,the optimum purification conditions were obtained：absorption time 5 h,elution time 30 min,ethanol concentration 80％,elution speed 1 ml/min,and obtain high purity of daidzin,genistin monomer.The antioxidation activity was analyzed,and the conclusion revealed that soybean isoflavones possessed superoxide radical and hydroxyl radical scavenging activities.Conclusion]The study has significance on development of soybean health food and natural medicine.