苦瓜多糖脱蛋白方法的比较研究

目的]寻找合适的苦瓜多糖脱蛋白方法。方法]比较了Seveage法、酶法、酶-Seveage结合法、三氯乙酸法以及盐酸法对苦瓜粗多糖的脱蛋白效果。结果]酶-Seveage结合法的脱蛋白效果最佳,木瓜蛋白酶用量为1.25%（V/V）,pH 6.5,温度60℃,时间为2 h,再用Se-veage试剂脱蛋白1次,蛋白质去除率达77.2%,多糖损失率仅为19.3%。结论]酶与Seveage结合法是一种有效的脱蛋白方法,此法可以较好地脱除游离蛋白,并使多糖损失较少。

Study on the Removal of Protein from Polysaccharides of Momordica charantia L.

Objective] The research aimed to seek the suitable deproteinization methods from Momordica charantia L.polysaccharides.Method] The deproteinization effects of Seveage method,enzyme method,enzyme-Seveage method,trichloroacetic acid method and hydrochloric acid method on M.charantia polysaccharides were compared.Result] The deproteinization effect of enzyme-Seveage method was the best,with the papain dose of 1.25%(V/V),pH 6.5 at 60 ℃ for 2 h.Then the polysaccharide solution was deproteinized again with Seveage agent and the removal rate of protein reached 77.2% and the loss rate of polysaccharide was 19.3%.Conclusion] The enzyme-Seveage method was an effective deproteinization method,with higher removal of dissociated protein and lower loss of polysaccharide.