| 白背毛木耳43-28胞外多糖的提取与纯化 |
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| 引用本文： | 张丹凤,郑丽珠,陈国平,潘裕添,郑仕栋.白背毛木耳43-28胞外多糖的提取与纯化[J].安徽农业科学,2010,38(31):17461-17462. |
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| 作者姓名： | 张丹凤 郑丽珠 陈国平 潘裕添 郑仕栋 |
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| 作者单位： | 漳州师范学院生物科学与技术系,福建漳州,363000;福建省漳州市农业局种植业管理站,福建漳州,363000 |
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| 基金项目： | 福建省教育厅项目(JA08159); 漳州师范学院科研基金项目 |
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| 摘 要： | 目的]对白背毛木耳43-28胞外多糖进行提取和纯化。方法]利用液体深层发酵进行培养，水提-醇析法分级提取胞外多糖，通过DEAE—cellulose52和SephadexG-200柱层析纯化胞外多糖。结果]提取得到白背毛木耳43—28胞外多糖，粗多糖得率为2．4％，总糖含量为54．67％；胞外多糖粗提物经DEAE—cellulose52柱层析后分成酸性和中性多糖，这2种多糖分别经SephadexG-200柱层析后。酸性多糖分离成大分子酸性多糖AP5和小分子酸性多糖AP62个组分，中性多糖只有AW1个组分。结论]成功提取得到白背毛木耳43-28胞外多糖并进行了纯化，为对白背毛木耳多糖的综合利用提供参考。
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| 关 键 词： | 白背毛木耳43-28 胞外多糖 提取 纯化 |
Extraction and Purification of Exopolysaccharide of Auricularia polytricha 43-28 |
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| ZHANG Dan-feng et al.Extraction and Purification of Exopolysaccharide of Auricularia polytricha 43-28[J].Journal of Anhui Agricultural Sciences,2010,38(31):17461-17462. |
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| Authors: | ZHANG Dan-feng |
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| Institution: | ZHANG Dan-feng et al(Department of Biological sciences , Technology,Zhangzhou Normal University,Zhangzhou,Fujian 363000) |
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| Abstract: | Objective] The aim was to study extraction and purification of Auricularia polytricha43-28 exopolysaccharide.Method] Auricularia polytricha 43-28 exopolysaccharide was extracted from fermented liquied by water extraction and alcohol precipitation,which was further purified by DEAE-cellulose 52 and Sephadex G-200.Result] The extracting rate of exopolysaccharide was 2.4%,in which the content of polysaccharide was 54.67%.After DEAE-cellulose 52,exopolysaccharide extraction was separated into two components:... |
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| Keywords: | Auricularia polytricha43-28 Exopolysaccharide Extraction Purification |
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