渤海黑牛H-FABP基因外显子2的序列测定与分析

目的]为改良渤海黑牛地方品种及种质资源的保护提供技术依据。方法]根据GenBank发表的牦牛心脏脂肪酸结合蛋白基因（H-FABP）的序列设计1对引物,利用PCR技术扩增出渤海黑牛H-FABP基因外显子2的DNA片段,测定其核苷酸序列并进行氨基酸序列的推导,同时将序列与牦牛、山羊、猪、人、小鼠和鸡的外显子2序列进行比较,并且进行系统发生树的构建。结果]渤海黑牛的H-FABP外显子2核苷酸序列和氨基酸序列与牦牛、山羊、猪、人、小鼠和鸡各物种间同源性分别为100%、97.1%、94.2%、89.0%、83.8%、79.8%,100%、98.2%、94.7%、86.0%、86.0%、77.2%。系统发生结果显示,系统发生树总体分为两支,鸡单独为一支;渤海黑牛、牦牛、山羊、猪、人、小鼠聚在一起成为另一支。结论]渤海黑牛H-FABP外显子2具有很强的保守性,H-FABP基因外显子2进化树符合物种进化规律。

Sequencing and Analyzing the Exon 2 of Heart Fatty Acid-Binding Protein (H-FABP) Gene of Bohai Black Cattle

Objective] The study aims to provide the technical basis for improving local variety of Bohai Black Cattle and resource protection.Method] According to Genbank combined with the H-FABP gene,sequences of yak,a pair of primers are designed.The Exon 2 of Heart Fatty Acid-Binding Protein(H-FABP) gene of Bohai Black Cattle is amplified and sequenced directly.Then the acquired sequence is aligned to further identify it and deduce the amino acid sequence with the homologous Exon 2 of H-FABP gene of other species...