库拉索芦荟组培中的褐变现象及防止

目的]研究库拉索芦荟组培中的褐变现象,寻求防止褐变的有效措施。方法]以库拉索芦荟茎尖、中部茎段和根茎为外植体,以MS为基本培养基,分别附加NAA0.1 mg/L和不同浓度的6-BA(1.0、1.5、2.0、3.0、4.0 mg/L),研究不同外植体类型、激素浓度以及培养温度、活性炭等因素对芦荟组培褐变的影响。结果]用库拉索芦荟茎尖做外植体,MS+6-BA2.0 mg/L+NAA0.1 mg/L做培养基,芦荟组培褐化程度较轻;培养基中加入活性炭500.0 mg/L,环境温度控制在25℃有利于减轻褐变。结论]茎尖是最佳的外植体,适宜的6-BA浓度(2.0 mg/L)可抑制褐变,添加活性炭和适宜的培养温度均能防止褐变死亡的发生。

Browning Phenomenon and its Prevention of Aloe barbadensis in Tissue Culture

Objective] The purpose was to study the browning phenomenon of Aloe barbadensis in tissue culture and seek for the effective measure of preventing browning.Method] With stem tips,middle stem segments and rhizomes of A.barbadensis as explants,MS was used as basic medium added NAA at 0.1 mg/L and 6-BA with different concentrations(1.0,1.5,2.0,3.0 and 4.0 mg/L) resp.to study the influences of factors such as different explant types, hormone concentrations,culture temperature and active carbon,etc.on the browning of A.barbadensis in tissue culture.Result] With the stem tips of A.barbadensis as explants,MS+6-BA 2.0 mg/L+NAA 0.1 mg/L as medium,the browning degree of A.barbadensis in tissue culture was lighter.Adding 500.0 mg/L active carbon into medium and controlling environmental temperature at 25 ℃ was in favor of lightening browning.Conclusion] The stem tip was the best explant and the suitable 6-BA concentration(2.0 mg/L) could inhibit browning. Both adding active carbon and the suitable culture temperature could prevent the occurrence of browning and death.