秸秆沼气发酵预处理微生物菌剂的筛选与特征研究

目的]研究添加微生物菌剂对秸秆沼气发酵产气效果的影响.方法]从沼气发酵液、树木土壤和堆肥中分离出多种微生物.通过初筛、复筛、桔抗试验等方法保留其中10种进行菌株配伍,选择产酶最高的4种微生物组合研制微生物菌剂.结果]4种产酶最高的微生物组合中12号纤维素酶活最高,为11.398 U/ml;8号漆酶晦活最高,为0.083 U/ml.6号微生物菌剂与秸秆的投料质量比为1∶50时,产气量最高.结论]该研究制备的复合微生物菌剂,作为秸秆沼气发酵预处理菌剂具有良好的应用前景.

Research on the Microbial Agents for Pretreatment of Biogas Fermentation

Objective] The research aimed to study the effect of agents for pretreatment of biogas fermentation by adding microbial agents.Method] A variety of microorganisms were isolated from methane fermentation broth,trees and compost in the soil.Ten kinds of them were retained through screening,rescreening and antagonistic experiments.Four kinds of microorganisms with highest enzyme activity were retained for the development of microbial agents.Result] The results showed that No.12 and No.8 groups of microorganisms owned the highest cellulase activity and laccase activity of 11.398 U/ml and 0.083 U/ml,respectively.Among all combinations,the gas production of No.6 was highest with No.6 agents and straw feed ratio of 1∶50.Conclusion] The compound microbial agents prepared in this study had excellent application prospects as biogas fermentation agents with straws.