| 膏桐叶片再生植株体系的建立 |
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| 引用本文： | 唐军荣,胥辉,马焕成,欧光龙,赵松浩,张金成.膏桐叶片再生植株体系的建立[J].安徽农业科学,2010,38(8):4190-4192,4243. |
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| 作者姓名： | 唐军荣 胥辉 马焕成 欧光龙 赵松浩 张金成 |
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| 作者单位： | 西南林学院西南地区生物多样性保育国家林业局重点实验室,云南昆明,650224;西南林学院西南地区生物多样性保育国家林业局重点实验室,云南昆明,650224;西南林学院西南地区生物多样性保育国家林业局重点实验室,云南昆明,650224;西南林学院西南地区生物多样性保育国家林业局重点实验室,云南昆明,650224;西南林学院西南地区生物多样性保育国家林业局重点实验室,云南昆明,650224;西南林学院西南地区生物多样性保育国家林业局重点实验室,云南昆明,650224 |
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| 基金项目： | 国家科技支撑计划项目(2007BAD32B02) |
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| 摘 要： | 目的]研究不同激素组合对离体叶片再生体系建立的影响。方法]选用6-BA与KT为分裂素,以无菌苗的离体叶片为外植体,在MS培养基中进行不定芽诱导,经增殖培养后,用生长素NAA、IBA促进生根,从而判断不同激素对不同外植体诱芽、增殖、生根的影响。结果]在MS培养基中,单独使用分裂素6-BA或者KT与生长素NAA的组合都不能较好地对叶片进行不定芽的诱导。在同时添加1.00mg/L的6-BA和KT以及0.03mg/L的NAA后对不定芽的诱导效果最好,诱导率达90%;加入0.50mg/L的6-BA、0.50mg/L的KT和0.02mg/L的NAA后,不定芽的增殖和伸长效果较好;在加入0.10mg/LNAA的MS培养基上生根效果最佳。结论]以MS培养基用适量浓度植物激素诱导膏桐离体叶片,可建立稳定的再生体系,为膏桐的组培扩繁体系提供了一种新的选择。
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| 关 键 词： | 膏桐 叶片 再生植株 组织培养 |
Research on the Establishment of the System of the Plantlet Regeneration from Jatropha curcas Leaf Culture |
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| TANG Jun-rong et al.Research on the Establishment of the System of the Plantlet Regeneration from Jatropha curcas Leaf Culture[J].Journal of Anhui Agricultural Sciences,2010,38(8):4190-4192,4243. |
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| Authors: | TANG Jun-rong |
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| Institution: | Southwest Forestry College;Key Laboratory of the Biodiversity Conservation in Southwest Region of the State Forestry Bureau;Kunming;Yunnan 650224 |
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| Abstract: | Objective] The effect of the different combinations of hormones on plantlet regeneration from Jatropha curcas leaf culture in vitro was studied.Method] The adventitious bud was induced from the leaf as explants cultured in vitro in MS medium containing 6-BA and KT as cytokinin and the adventitious buds after proliferation culture were rooted in the medium with NAA and IBA as cellular auxin.The effect of the different hormones or their combination on the induction of adventitious bud,proliferation and root... |
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| Keywords: | Jatropha curcas Leaf Plantlet Tissue culture |
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