蜡蚧轮枝菌对桃蚜的室内毒力测定研究 |
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引用本文: | 刘春来,王爽,李新民,刘兴龙,夏吉星,杨帆,马玲.蜡蚧轮枝菌对桃蚜的室内毒力测定研究[J].安徽农业科学,2013(36):13904-13906. |
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作者姓名: | 刘春来 王爽 李新民 刘兴龙 夏吉星 杨帆 马玲 |
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作者单位: | 东北林业大学林学院,黑龙江哈尔滨150040;黑龙江省农业科学院植物保护研究所,黑龙江哈尔滨150086;黑龙江省农业科学院植物保护研究所,黑龙江哈尔滨,150086;东北林业大学林学院,黑龙江哈尔滨,150040 |
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基金项目: | 国家国际科技合作专项项目(2012DFR30810). |
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摘 要: | 目的]筛选对桃蚜侵染致病力强的蜡蚧轮枝菌菌株,为该菌的进一步开发利用奠定基础.方法]采用波特喷雾塔测定法,测定来源于俄罗斯的6株蜡蚧轮枝菌菌株对桃蚜的侵染致死情况.结果]高浓度处理(>1×106孢子/ml),多数菌株在第5、6天达到死亡高峰;第7天结束观察时,所测定各菌株的最高浓度处理(1×108孢子/ml)若蚜校正死亡率均达到95%以上.V3、V4菌株在该浓度下致死中时间分别为4.25和4.11 d.结论]参试菌株对桃蚜均表现出较高的致病性,V3、V4菌株为更优良的备用菌株.
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关 键 词: | 蜡蚧轮枝菌 桃蚜 波特喷雾塔 校正死亡率 致死中时间 |
The Toxicity Bioassayed of Verticillium lecanii Strains against Myzus persica in Laboratory |
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Institution: | LIU Chun-lai( 1.College of Forestry, Northeast Forestry University, Harbin, Heilongjiang 150040;) |
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Abstract: | Objective] The study aimed to screening high virulence of Verticillium lecanii to Myzus persica for further development and utilization.Method] Six Verticillium lecanii strains from Russia were bioassayed to Myzus persica by Potter Spray Tower.Result] The results showed that Myzus persica reached the peak of death with the treatment of high concentration (> 1 × 106 spores / ml) of the majority of strains after the five or six days,At the end of the seventh day observation,the mortality of Myzus persica was above 95% with the highest concentrations(1 × 108 spores / ml)of each strain.LT50 of V3 and V4 stain was 4.25 and 4.11 days with the highest concentrations(108 spores/ml) respectively.Conclusion] Six Verticillium lecanii strains showed higher pathogenicity on Myzus persicae,V3 andV4 stains were more excellent and alternate strains. |
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Keywords: | Verticillium lecanii Myzus persica Potter spray tower Mortality LT50 |
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