干旱胁迫对分蘖期转基因水稻抗旱性生理生化指标的影响

目的]通过比较野生型日本晴株系水稻WT和OsCAS基因过表达株系水稻777在分蘖期的抗旱性生理生化指标,分析OsCAS基因高表达株系水稻的抗旱能力。方法]用10%和15%的PEG模拟干旱胁迫,测定2种株系水稻的CAT、POD和SOD活性及MDA和Pro含量。结果]在10%PEG处理下,WT和777的3种保护酶活性均有增加;在15%PEG处理下,WT的3种保护酶活性明显降低,777的3种保护酶却能够保持相对较高的活性;此外,WT和777的MDA和Pro含量都随干旱胁迫程度的加深而增加,在同一胁迫条件下WT的MDA和Pro含量要明显高于777的。结论]OsCAS基因高表达株系777在分蘖期较WT对干旱有较强的抗性,特别是在严重干旱胁迫(15%PEG)时优势尤为明显。

Effects of Drought Stress on Physiological and Biochemical Indices of Drought-resistance in Genetically Modified Rice Tillering Stage

Objective] To study the drought resistance of 777（a kind of rice with OsCAS gene overexpression）,we compared the physiological and biochemical indices of drought-resistance between the WT（a kind of wild rice,Nipponbare rice） and 777.Method] Two different drought resistant varieties were treated by 10% and 15%PEG.Their CAT,SOD and POD activity and Pro and MDA contents were measured.Result] The results indicated that under 10% PEG treatment,the CAT,SOD and POD activity were increased in both WT and 777.Under 15% PEG treatment,the CAT,SOD and POD activity decreased in WT but maintained relatively higher level in 777.In the two different varieties,the MDA and Pro content augmented with PEG concentration increasing.There were more Pro and MDA in WT than 777 under the same drought stress.Conclusion] In tillering stage,the 777 has a stronger ability to resistant the drought stress than the WT,especially under serious drought stress（15%PEG）.