长丝裂腹鱼MyoD1基因的克隆及序列分析

目的]克隆长丝裂腹鱼的MyoD1基因,并对其进行序列分析。方法]根据鲤鱼(Cyprinus carpio)的MyoD1基因序列设计引物,采用RT-PCR方法对长丝裂腹鱼MyoD1的全长cDNA序列进行扩增,并对该基因编码氨基酸的理化性质及同源性进行预测和分析。结果]试验获得了包括完整ORF的长丝裂腹鱼MyoD1基因,该序列长957 bp,编码274个氨基酸,具有MRFs家族基因的典型性碱性bHLH结构;该MyoD1序列与齐口裂腹鱼、鲤鱼、斑马鱼和虹鳟等的MyoD1序列同源性较高,与人、猪、牛等哺乳动物的同源性较低。结论]该研究为长丝裂腹鱼的肌肉发育和肉质特性形成的分子机制研究提供了基础数据,同时为青藏高原冷水鱼资源的利用与保护提供了指导意义。

Clone and Sequence Analysis of MyoD1 gene in Schizothorax dolichonema

Objective] The paper was to clone and analyze the sequence of MyoD1 gene in Schizothorax dolichonema.Method] Primers were designed according to the MyoD1 gene sequence of Cyprinus carpio,and the full-length cDNA sequence of MyoD1 in S.dolichonema was amplified by RT-PCR method.The homology,physical and chemical properties of the encoded amino acid were analyzed and predicted.Result] The MyoD1 gene of S.dolichonema including a complete ORF was cloned,and the sequence was 957 bp with typical alkaline bHLH structure of MRFs family genes,encoding 274 amino acids.The homology of MyoD1 sequence in S.dolichonema was higher compared with Schizothorax prenanti,Cyprinus carpio,Danio rerio and Oncorhynchus mykiss,but it was lower in some mammals and poultry.Conclusion] The study provided basic data for the studies of muscle growth and molecular mechanism of meat quality characteristics’ formation,also provided guidance for the utilization and protection of cold water fish resources Qinghai-Tibetan plateau.