鲜切马铃薯实时荧光定量PCR分析中内参基因的选择

目的]选择鲜切马铃薯实时荧光定量PCR(qRT-PCR)分析中合适的内参基因。方法]以受到高温、光照等胁迫的鲜切马铃薯为材料,应用qRT-PCR技术,分析18S rRNA、GAPDH、Actin和EF1a 4个常用内参基因的表达情况。结果]经GeNorm软件分析发现,当利用qRT-PCR分析比较鲜切马铃薯中的基因表达差异时,可选择Actin作为校正内参基因。结论]该研究为进一步开展鲜切马铃薯分子生物学研究奠定了方法学基础。

Reference Genes Selection for Real-time Fluorescence Quantitative PCR in Fresh-cut Potato

Objective] The paper was to select suitable reference genes for the real-time fluorescence quantitative PCR（qRT-PCR） in freshcut potato.Method] Using the fresh-cut potatoes under high temperature,illumination and other stress as materials,the expression of four commonly used reference genes 18S rRNA,GAPDH,Actin and EF1a was analyzed by qRT-PCR technology.Result]Through the analysis of GeNorm software,it found that when the qRT-PCR was used to analyze and compare the gene expression differences of fresh-cut potatoes,the Actin could be chosen as the calibration reference gene.Conclusion]The study laid a methodology foundation for the further development of molecular biological study in fresh-cut potato.