木耳菌糠对元蘑胞外酶活性的影响

目的]筛选利用木耳菌糠栽培元蘑的最佳配方。方法]设计4种配方，选取元蘑菌丝生长期、原基形成期和子实体发育期等阶段，测定其羧甲基纤维素酶（CMC）、半纤维素酶（HC）、淀粉酶（AMY）、漆酶（LAC）4种胞外酶的活性。结果]在不同配方中，元蘑菌丝体所分泌的CMC、HC、AMY和LAC酶活具有明显的差异，但同种酶的活性在不同配方中变化趋势基本一致；经过显著性分析，配方1、配方2和配方3胞外酶活性无显著差异，但配方4的酶活显著低于其他3种配考。结论]在胞外酶水平上，配方1、配方2和配方3用来栽培元蘑都是可行的。从经济成本考虑，配方3（茵糠含量为36％）为最佳配方。

Effect of Waste Material of Auricularla auricular on Hohenbuehelia serotina Extracellular Enzyme Activities

Objective ] The research aimed to select the optimal formulation to cultivate Hohenbuehelia serotina with waste material of Auricu- laria auricular. Method ] 4 formulations were designed and mycetium growth phase, primordium formation phase and fruiting body growth phase were chosen, the activities of CMC, HC, AMY and LAC were determined. Result] In different formulations, CMC, HC, AMY and LAC that H. serotina mycelium secreted had significant differences, but the same enzyme activity had the consistent tendency in different for- mulations. After significance analysis, extracellular enzyme activities in formulation 1,2,3 had no significant differences, but they were signifi- cantly lower in formulation 4. Conclusion ] On the extracellular enzyme level, cultivating H. serotina with formulation 1,2,3 was feasible. Considering economic costs, formulation 3 （waste material of 36% ） was optimal.