“童子1号”草莓组培快繁技术研究

目的]探讨低成本下草莓试管苗的快繁技术。方法]以"童子1号"草莓匍匐茎的顶芽为试材,以附加不同浓度BA、NAA的MS培养基诱导和增殖草莓小苗,记录组培苗的诱导与增殖情况。带顶芽的茎段基部蘸不同浓度的IBA、NAAI、AA,观察生根情况。结果]草莓低成本下最适诱导分化培养基为MS+BA 0.5 mg/L。BA明显促进芽的增殖,KT会影响芽的增殖率。最优的增殖培养基为MS+BA 0.5 mg/L。3种不同浓度激素处理组培苗的生根差别不大。组培苗瓶外生根蘸取的最佳生长调节剂浓度为IBA 800 mg/L。试管生根苗直接移栽的成活率高达99%。结论]所有培养基均以3%食用白砂糖代替蔗糖,以自来水代替蒸馏水,大大降低了培养成本,每株成本下降。

Research on Regeneration Technique of Strawberry"Tongzi 1"

Objective] The study was to explore the rapid propagation technique of strawberry seedlings in vitro with low cost.Method] With the top buds on the stolon of tongzil as material,the seedling of strawberry was induced and multiplied in MS medium mixed BA、NAA with different concentration and the effect of its induction and multiplication was investigated.The rooting effect of the base of the stem with top bud shocking in different concentration of IBA,NAA and IAA was studied.Result] The optimum medium for differentiation was MS + BA 0.5 mg/L in low cost condition.BA could improved the propagation of shoot,while KT could decrease its propagation.The optimum medium for propagation was MS+BA0.5 mg/L.The effect of rooting during 3 plant growth regulator with different concentration had a slight difference.The optimum concentration of soaking root was IBA 800 mg/L out of bottle.The directly transplant living rate of seedling researched 99%.Conclusion] The cost could be decreased markedly with 3% edible sugar instead of sucrose and tap water instead of distilled water,and the cost of media per plant could be decreased to 75%.