GC-MS法测定猪肝、猪尿中盐酸克伦特罗残留量的研究

采用乙酸乙酯提取、利用盐酸克伦特罗易溶于酸性溶液的特点进行液液分配、固相萃取相结合的方法提取组织中残留的盐酸克伦特罗,用双三甲基硅基三氟乙酰胺(BSTFA)衍生化,毛细管气相色谱/质谱联用(选择离子检测模式,检测离子M/Z为862、122、622、77)对衍生化物进行检测分析。结果显示,不同添加浓度的盐酸克伦特罗在猪肝中的平均回收率在85%~95%,批内变异系数在4.8%~8.2%,实际检出限:猪肝为0.30μg/kg,猪尿为0.25μg/L。

Detection of Clenbuterol Hydrochloride Residue in Pig Liver and Pig Urine Using Gas-Chromatography Mass-Spectrometry

A confirmatory method was presented for the detection of clenbuterol hydrochloride residue tissues by gas chromatography-mass spectrometry(Gc-Ms).The method was based on the fact that clenbuterol hydrochloride was easily dissolved in acidic solution.So we can get the extraction from ethyl acetate by liquid-liquid distribution coupled with solid phase extraction.The derivatation was performed with Bis-(Trimethylsilyl)-Trifluoroacetamide(BSTFA)and detected by capillary gas chromatography/Mass spectrometry in selected ion monitoring mode(the detective ions are at M/Z 86,212,262,277).The results showed that the average recovery of clenbuterol hydrochloride changed from 85% to 95% and its coefficient of variation in batch assay ranged between 4.8% and 8.2%.The limit of detection in pig liver and pig urine was 0.30 μg/kg and 0.25 μg/L,respectively.