| 壳寡糖类物质对烟草TMV的抑制及防御酶系的影响 |
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| 引用本文： | 马镝,吴元华,赵秀香.壳寡糖类物质对烟草TMV的抑制及防御酶系的影响[J].安徽农业科学,2006,34(18):4659-4660,4662. |
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| 作者姓名： | 马镝 吴元华 赵秀香 |
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| 作者单位： | 沈阳农业大学,辽宁,沈阳,110161 |
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| 基金项目： | 沈阳农业大学校科研和教改项目 |
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| 摘 要： | 在接种烟草花叶病毒(TMV)前几天,用自制的壳寡糖溶液处理心叶烟(Nicotiana glutinosa)和烟草品种NC89(Nicotiana tobaccumcvNC89),测定其在烟草上对TMV的抑制作用及引起的寄主体内防御酶系变化。结果表明:壳寡糖处理后,在心叶烟上对TMV的病斑抑制率在第10天达最高,为79.10%;在烟草品种NC89上对TMV的防效在第10天达最高,为32.41%。同时,壳寡糖处理可导致烟草品种NC89叶片的PAL、POD、PPO、SOD活性不同程度提高,可溶性蛋白含量增加。表明壳寡糖类物质可以诱导烟草对TMV的抗性,提高烟草的免疫能力。
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| 关 键 词： | 壳寡糖 烟草花叶病毒 防御酶系 |
| 文章编号： | 0517-6611（2006）18-4659-02 |
| 收稿时间： | 2006-07-24 |
| 修稿时间： | 2006-07-24 |
Inhibition of Chitosan oligosaccharides to TMV and its Effect on Defensive Enzyme of Tobacco |
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| MA Di,et al.Inhibition of Chitosan oligosaccharides to TMV and its Effect on Defensive Enzyme of Tobacco[J].Journal of Anhui Agricultural Sciences,2006,34(18):4659-4660,4662. |
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| Authors: | MA Di |
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| Institution: | Shenyang Agricultural University, Shenyang, Liaoning 110161 |
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| Abstract: | Before inoculation with TMV some days,the tobacco treated with chitosan-oligosaccharides was tested in inhibition to TMV and its effect on defensive enzymes.The results indicated that the inhibition rate was up to 79.10% in Nicotiana glutinosa on 10~(th) day and the control effect was 32.41% in Nicotiana tobaccum cv.NC89 on 10~(th)day.The activity of PAL,POD,PPO,SOD and the content of the solubility of protein was increased with various degrees.So it can prove that the chitosan-oligosaccharides can induce the resistance of tobacco to TMV,thereby it can strengthen the immunity of tobacco. |
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| Keywords: | Chitosan-oligosaccharides TMV Defensive enzyme |
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