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| 洛克沙胂暴露对斑马鱼的急性毒性及其组织中HSP70的表达定位 | |
| 引用本文： | 朱爱华,张雨梅,毛伟.洛克沙胂暴露对斑马鱼的急性毒性及其组织中HSP70的表达定位[J].安徽农业科学,2010,38(5):2351-2353. |
| 作者姓名： | 朱爱华 张雨梅 毛伟 |
| 作者单位： | 1. 江苏省东辛农场畜牧兽医站,江苏连云港,222248 2. 扬州大学兽医学院,江苏扬州,225009 3. 江苏省连云港市特教中心,江苏连云港,222000 |
| 摘 要： | 目的]对洛克沙胂的水生生态安全评价、畜禽粪便管理以及HSP70在斑马鱼应激状态下的保护作用的研究提供科学依据。方法]以斑马鱼为试验生物,进行洛克沙胂暴露对斑马鱼96 h的急性毒性试验,并对洛克沙胂暴露下斑马鱼组织（鳃、肝脏、性腺）中HSP70的表达定位进行了免疫组织化学初步研究。结果]洛克沙胂对斑马鱼的急性毒性较低,96 hLC50为249.80 mg/L;在浓度100mg/L洛克沙胂暴露下,HSP70在斑马鱼的鳃、肝脏、性腺3种组织中有特异性表达,主要定位在鳃小片的基底膜、鳃弓和鳃丝上皮细胞及红细胞的胞质中,性腺的基质细胞中,肝脏的静脉血管四周和红细胞胞质中。结论]洛克沙胂对于斑马鱼的毒性属于低毒范围。 |
| 关 键 词： | 洛克沙胂 斑马鱼 急性毒性 HSP70 免疫组化 |
Acute Toxic Experiment of Roxsarsone on Zebrafish and HSP70 Location in the Tissues of Zebrafish Induced by Roxsarsone |
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| ZHU Ai-hua et al.Acute Toxic Experiment of Roxsarsone on Zebrafish and HSP70 Location in the Tissues of Zebrafish Induced by Roxsarsone[J].Journal of Anhui Agricultural Sciences,2010,38(5):2351-2353. | |
| Authors: | ZHU Ai-hua |
| Institution: | Animal Husbandray and Veterinary Station of Dongxin Farm;Lianyungang;Jiangsu 222248 |
| Abstract: | Objective]The research aimed to provide scientific references for the study on the safety evaluation on aquatic ecosystem of roxasone,animal manure arrangement,the protection of HSP70 on zebrafish.Method]The 96 h acute toxic experiment of roxasone was carried out using zebrafish as test organism,and the variation of HSP70 of zebrafish's gills,gonads and liver tissues was studied by immunochemistry.Result]The results showed that the 96 h LC50 value of roxarsone was 249.80 mg/L.HSP70 located specially in t... |
| Keywords: | Roxsarsone Zebrafish Acute toxicity HSP70 Location Immunochemistry |
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