水果型番木瓜组织培养的研究

目的]研究水果型番木瓜的组织培养。方法]以经预处理的种子为试材,通过MS基本培养基附加不同浓度的6-BA和IBA,对水果型番木瓜组培快繁技术进行了研究。结果]无菌条件下用75%酒精均匀擦拭水果型番木瓜果皮表面,取出种子,用无菌水冲洗3次是较好的灭菌方法,种子污染率仅为2.52%;水果型番木瓜种子经1000mg/LGA3＋1mg/L6-BA（等体积混合）浸泡18h后接种于MS培养基中培养,种子发芽率、胚芽长度、胚根长度和苗高达到较高值,分别为68.42%、2.25cm、0.80cm和1.52cm,幼苗整体生长较好;继代增殖培养基为MS＋0.5mg/L6-BA＋0.1mg/LIBA时增殖系数最高（7.92）,幼苗长势较好;组培苗玻璃化随着光照强度的增强而呈减弱趋势,3000lx处理下玻璃化苗比率最低（3.21%）。结论]该研究为水果型番木瓜的组培快繁研究提供了参考。

Tissue Culture of Mini-Papaya

Objective]The aim was to study the tissue culture of mini-Papaya.Method]The pretreatment seeds of mini-Papaya were cul-tured in the MS medium containing 6-BA and IBA of different densities for rapid propagation.Result]In the condition of aseptic strain,the surface of mini-Papaya peel was uniformly wiped by 75% alcohol,then seeds were removed and washed by aseptic water for 3 times,which was the best sterilization method,and the pollution rate of seed was only 2.52% .After seeds which had been soaked by the equivolume mix-solution of 1 000 mg/L GA3 and 1 mg/L 6-BA for 18 h were further purified in MS medium,the germination rate of seed,the length of germ and radical and the height of seedling were 68.42% ,2.25 cm,0.80 cm and 1.52 cm,respectively,furthermore the total situation of seedling growth was better.When subculture multiplication medium was MS + 0.5mg/L 6-BA + 0.1 mg/L IBA medium,proliferation coefficient of sub-culture multiplication reached the highest (7.92) ,and the seedings grew better.The ratio of vitrified shoots decreasing with the increase of light intensity could reach the lowest level (3.21% ) under the light intensity of 3 000 lx.Conclusion]The research provides reference for studies on tissue culture and rapid propagation of mini-Papaya.