培养条件对嗜热芽孢杆菌HU1合成几丁质降解酶及脱乙酰基酶的影响

目的]研究培养条件对嗜热芽孢杆菌HU1产酶的影响,为工业化发酵生产酶及壳寡糖的制备提供依据。方法]采用摇瓶培养方式,对嗜热芽孢杆菌HU1产几丁质降解酶及脱乙酰基酶的培养条件进行研究。结果]最佳诱导碳源为粉末几丁质,其最适添加量为3.5%;最佳氮源为酵母粉,最适添加量为1.0%;初始pH值为6.0时,有利于产酶;Cu2＋对酶的合成表现出明显的抑制作用;而Ca2＋则能有效增加产酶能力。Mg2＋及Zn2＋对嗜热芽孢杆菌HU1 2种酶的合成无显著影响;培养72 h,产酶量达到最高。以粉末几丁质为底物,对粗酶液的水解产物进行了分析,其12 h的水解产物主要集中于分子量为六糖以下的壳聚糖。结论]利用粗酶液制备小分子壳寡糖工艺流程简单,为后期进一步开发保健品、生物农药、饲料添加剂、食品添加剂等奠定了基础。

Effects of Culture Conditions on the Synthesis of Chitinase and Chitin Deacetylase from Thermophilic Bacillus sp. HU1

Objective] The aims were to investigate the effects of culture conditions on the enzyme production by Thermophilic Bacillus sp.Hu1 and provide basis for fermentation of enzyme and preparation of chitosan oligosaccharide.Method] The shake flask culture was employed to research the culture conditions of Thermophilic Bacillus sp.for producing Chitinase and Chitin Deacetylase HU1.Result] The optimum carbon source was powder chitinase with the optimum addition of 3.5%;while yeast power was the optimum nitroge...