太湖稻区32个水稻品种DNA指纹图谱的构建及遗传多样性分析

目的]采用SSR标记构建太湖稻区16个常规粳稻品种和16个杂交水稻品种DNA指纹图谱并进行遗传多样性分析.方法]以筛选出的12对多态性高、稳定性好且在染色体上分布均匀的引物作为核心引物,构建太湖稻区32个主要栽植水稻品种DNA指纹图谱,以NTSYS-PCV2.10软件分析遗传多样性.结果]12对SSR引物在32份材料中共扩增出了47个等位基因,平均每对引物4.7个,变幅2～6个;12对引物的多态性频率（FP）平均值为0.627,变幅0.266～0.833;以遗传相似系数0.74为阚值可将供试32个水稻品种分成4类.结论]太湖稻区32个水稻品种遗传多样性相对狭窄.

Construction of Fingerprint and Analysis of Genetic Diversity with SSR Markers for Thirty-Two Rice Cultivars in Taihu Lake Aera

Objective ] The aim of this study was to construct a DNA fingerprinting database of 32 rice cultivars in Taihu lake area, and thegenetic diversity was analyzed based on simple sequence repeats（SSR）. Method] Twelve evenly distributed SSR primer pairs with high poly-morphisms and good repeatability were successfully screened to construct the fingerprinting database. Result] Among the 32 varieties, 12primer pairs had 47 polymorphic locis, and 4.7 polymorphic locis were detected by each SSR primer pair on an average with the range from 2to 6. Results show that, SSR markers are suitable for using in construction of rice DNA fingerprint with polymorphism differences between theselected varieties. The genetic deversity analyzed by the software of NTSYS-pc V2.10 indicated that, 32 rice varieties can be divided into 4types based on the genetic similarity coefficient of 0.74 threshold. Conclusion] The genetic diversity of 32 rice cultivars in Taihu lake area isrelatively narrow.