| 宁夏枸杞甜菜碱乙醛脱氢酶（LbBADH2）的克隆与序列分析 |
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| 引用本文： | 樊云芳,陈晓军,曹有龙.宁夏枸杞甜菜碱乙醛脱氢酶（LbBADH2）的克隆与序列分析[J].安徽农业科学,2014,42(36):12798-12802. |
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| 作者姓名： | 樊云芳 陈晓军 曹有龙 |
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| 作者单位： | 1. 国家枸杞工程技术研究中心,宁夏银川,750002
2. 宁夏农业生物技术重点实验室,宁夏银川,750002 |
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| 基金项目： | 国家863计划，国家自然科学基金项目 |
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| 摘 要： | 以宁夏枸杞为试验材料,利用同源克隆技术,设计简并引物,克隆得到一个新的甜菜碱醛脱氢酶基因.该基因全长cDNA为1 527个碱基,编码508个氨基酸,编码区两侧翼分别具有5＇UTR （47 bp）和3＇UTR（129 bp）.在推导的氨基酸序列中,含有醛脱氢酶所具有的高度保守的十肽（VrlElGGKSP）以及与酶功能有关的半胱氨酸残基（C）.进化分析表明,它与番茄甜菜碱醛脱氢酶亲缘关系最近,与其他藜科植物亲缘关系较远.该基因的获得有助于理解枸杞这一盐生植物耐盐机理,为培育新的耐盐枸杞奠定理论基础.
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| 关 键 词： | 宁夏枸杞 同源克隆 BADH2 |
Cloning and Sequence Analysis of LbBADh2 in Lycium barbarum |
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| FAN Yun-fang
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CHEN Xiao-jun
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CAO You-long.Cloning and Sequence Analysis of LbBADh2 in Lycium barbarum[J].Journal of Anhui Agricultural Sciences,2014,42(36):12798-12802. |
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| Authors: | FAN Yun-fang CHEN Xiao-jun CAO You-long |
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| Institution: | FAN Yun.fang, CHEN Xiao-jum, CAO You-long ( 1. National EIgineering Research Center for Wotiberry, Yinchuan, Ningxia 750002; 2. Key Lab of Agricultural Biotechnology of Ningxia, Yinchuan, Ningxia 750002) |
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| Abstract: | A new gene of Betaine Aldehyde Dehydrogenase （BADH）, named LbBADH2, was obtained by RACE technology in Lycium barba-rum. The full cDNA of LbBADH is consist of 1527 base pairs and encode 508 aa. , 5＇UTR （47 bp） and 3＇UTR （129 bp ） locating both side ofit. A high conserved decapeptide sequence ＇ VTLELGGKSP＇ and Cys, which are related to the activity of enzyme, are found in putative aminoacid among all BADH. The evolutional analysis showed that LbBADH2 is closer with So/attain lycopersicum than others plant in genetic relation-ship. The obtaining of LbBADH2 will help understanding the mechanism of salt tolerance and lay theoretical foundation for developing new sah-tolerance variety in Lycium barbarum. |
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| Keywords: | Lycium barbarum Homo-cloning BADH2 |
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